Listar por autor "937dfce0-7c9a-434c-b83d-12595798aa1e"
Mostrando ítems 1-12 de 12
-
A potent antihemorrhagin in the serum of the non-poisonous water snake Natrix tessellata: isolation, characterization and mechanism of neutralization
Borkow, Gadi; Gutiérrez, José María; Ovadia, Michael (1994-12)The main natural antihemorrhagic factor (NtAH), which inhibits the hemorrhagic activity of Bothrops asper snake venom, was isolated from the serum of the non-poisonous water snake Natrix tessellata by ammonium sulfate ... -
Activity of hemorrhagic metalloproteinase BaH-1 and myotoxin II from Bothrops asper snake venom on capillary endothelial cells in vitro
Lomonte, Bruno; Gutiérrez, José María; Borkow, Gadi; Ovadia, Michael; Tarkowski, Andrej; Hanson, Lars Ake (1994-04)In vivo, hemorrhagic toxins isolated from snake venoms cause a disorganization of the basal lamina of capillaries, with a concomitant degenerative process of endothelial cells. In this study we investigated the effects of ... -
Effect of various Viperidae and Crotalidae snake venoms on endothelial cells in vitro
Borkow, Gadi; Lomonte, Bruno; Gutiérrez, José María; Ovadia, Michael (1994)The effect of various crotalid and viperid venoms at 10, 50 and 100 μg/ml was examined on bovine and murine endothelial cells in vitro. The venoms caused the cells to lose their processes, leading to the appearance of ... -
Immunological studies on BaH1 and BaPI, two hemorrhagic metalloproteinases from the venom of the snake Bothrops asper
Rucavado Romero, Alexandra; Borkow, Gadi; Ovadia, Michael; Gutiérrez, José María (1995-08)No immunological cross-reactivity was observed between BaH1 and BaP1, two hemorrhagic metalloproteinases isolated from B. asper venom, by gel immunodiffusion, Western blotting and neutralization studies. Cross-reactivity ... -
In vitro activity of BaH1, the main hemorrhagic toxin of Bothrops asper snake venom on bovine endothelial cells
Borkow, Gadi; Gutiérrez, José María; Ovadia, Michael (1995-10)Incubation of BaH1, the main hemorrhagic toxin purified from the venom of Bothrops asper, with endothelial cells caused the appearance of spaces among the cells. This effect became more noticeable with increasing hemorrhagin ... -
Inhibition of the hemorrhagic activity of Bothrops asper venom by a novel neutralizing mixture
Borkow, Gadi; Gutiérrez, José María; Ovadia, Michael (1997-06)This study screened 25 sera, 19 synthetic products and five antivenoms obtained after immunization for their ability to neutralize the hemorrhagic activity of venom from the snake Bothrops asper. Among the sera screened, ... -
Inhibition of toxic activities of Bothrops asper venom and other crotalid snake venoms by a novel neutralizing mixture
Borkow, Gadi; Gutiérrez, José María; Ovadia, Michael (1997-12)The majority of snake bites in Central America are caused by Bothrops asper, whose venom induce complex local effects such as myonecrosis, edema and especially hemorrhage. These effects are only partially neutralized by ... -
Isolation and characterization of a metalloproteinase with weak hemorrhagic activity from the venom of the snake Bothrops asper (terciopelo)
Gutiérrez, José María; Romero, Marjorie; Díaz Oreiro, Cecilia; Borkow, Gadi; Ovadia, Michael (1995-01)A metalloproteinase, named BaP1, was purified to homogeneity from the venom of Bothrops asper (Pacific region) of Costa Rica by ion-exchange chromatography on CM-Sephadex and gel filtration on Sephacryl S-200. The enzyme ... -
Isolation and characterization of synergistic hemorrhagins from the venom of the snake Bothrops asper
Borkow, Gadi; Gutiérrez, José María; Ovadia, Michael (1993-09)Three hemorrhagic factors (BaH1, BH2 and BH3) were isolated from the venom of Bothrops asper by gel filtration on Sephacryl S-200, DEAE-Sepharose chromatography, metal chelate affinity chromatography and hydrophobic ... -
Pathological changes induced by BaH1, a hemorrhagic proteinase isolated from Bothrops asper (Terciopelo) snake venom, on mouse capillary blood vessels
Moreira Carmona, Lisela; Borkow, Gadi; Ovadia, Michael; Gutiérrez, José María (1994-08)The pathological changes induced in capillaries by BaH1, a hemorrhagic metalloproteinase isolated from the venom of Bothrops asper, were studied after i.m. injection in mouse gastrocnemius. Hemorrhage was observed ... -
Skeletal Muscle Necrosis and Regeneration after Injection of BaH1, A Hemorrhagic Metalloproteinase Isolated from the Venom of the Snake Bothrops asper (Terciopelo)
Gutiérrez, José María; Romero, Marjorie; Núñez, Javier; Chaves Mora, Fernando; Borkow, Gadi; Ovadia, Michael (1995-02)The effects of BaH1, a hemorrhagic metalloproteinase isolated from Bothrops asper venom, on mouse gastrocnemius muscle was investigated. The toxin induced severe hemorrhage within minutes after injection. Groups of necrotic ... -
Ultrastructural alterations in mouse capillary blood vessels after experimental injection of venom from the snake Bothrops asper (Terciopelo)
Moreira Carmona, Lisela; Gutiérrez, José María; Borkow, Gadi; Ovadia, Michael (1992-10)Histological and ultrastructural alterations in capillary blood vessels were studied at various time intervals after im injection of 50 micrograms of Bothrops asper snake venom in mouse gastrocnemius muscle. Hemorrhage was ...