Venomics of new world pit vipers: genus-wide comparisons of venom proteomes across Agkistrodon
Ureña Diaz, Juan M.
Mora Obando, Diana
Fry, Bryan G.
Gutiérrez, José María
Gibbs, H. Lisle
Sovic, Michael G.
Calvete Chornet, Juan José
MetadataShow full item record
Wereport a genus-wide comparison of venomproteomevariation acrossNewWorld pit vipers in the genus Agkistrodon. Despite the wide variety of habitats occupied by this genus and that all its taxa feed on diverse species of vertebrates and invertebrate prey, the venom proteomes of copperheads, cottonmouths, and cantils are remarkably similar, both in the type and relative abundance of their different toxin families. The venoms from all the eleven species and subspecies sampled showed relatively similar proteolytic and PLA2 activities. In contrast, quantitative differences were observed in hemorrhagic and myotoxic activities in mice. The highest myotoxic activity was observed with the venoms of A. b. bilineatus, followed by A. p. piscivorus, whereas the venoms of A. c. contortrix and A. p. leucostoma induced the lowest myotoxic activity. The venom of Agkistrodon bilineatus showed the highest hemorrhagic activity and A. c. contortrix the lowest. Compositional and toxicological analyses agree with clinical observations of envenomations by Agkistrodon in the USA and Central America. A comparative analysis of Agkistrodon shows that venom divergence tracks phylogeny of this genus to a greater extent than in Sistrurus rattlesnakes, suggesting that the distinct natural histories of Agkistrodon and Sistrurus clades may have played a key role in molding the patterns of evolution of their venom protein genes.
External link to the itemdoi: 10.1016/j.jprot.2013.10.036
artículo (arbitrado) -- Universidad de Costa Rica. Instituto de Investigaciones Clodomiro Picado, 2013. Este documento es privado debido a limitaciones de derechos de autor.
The following license files are associated with this item: