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In vitro evaluation of nematophagous fungi of Costa Rica rice-growing regions against the root-knot Meloidogyne javanica
Evaluación in vitro de hongos nematófagos en zonas arroceras de Costa Rica contra el nematodo agallador Meloidogyne javanica
dc.creator | Peraza Padilla, Walter | |
dc.creator | Orozco Aceves, Martha | |
dc.creator | Esquivel Hernández, Alejandro | |
dc.date.accessioned | 2015-06-09T22:41:58Z | |
dc.date.available | 2015-06-09T22:41:58Z | |
dc.date.issued | 2014-12-01 13:43:06 | |
dc.identifier.citation | http://revistas.ucr.ac.cr/index.php/agrocost/article/view/17271 | |
dc.identifier.issn | ||
dc.identifier.uri | https://hdl.handle.net/10669/13997 | |
dc.description.abstract | Parasitism in vitro of 14 isolates of nematophagous fungi (6 Trichoderma sp., 4 Paecilomyces sp., 2 Fusarium oxysporum and 2 Monacrosporium sp.) was evaluated againts juveniles and eggs of the root-knot nematode, Meloidogyne javanica. The fungi, from three rice-producing regions of Costa Rica (Central Pacific, Atlantic Huetar and Chorotega), were isolated by the sprinkling technique, during April 2008 to August 2009. A completely randomized design with 10 replicates in water agar (WA) per fungus was used. A suspension of 1 to 1.5x10 6 conidia (cfu.ml -1 ) per Petri dish was inoculated, and during the fourth day was added a suspension of 0.5 ml with 150 juvenile and 100 eggs of M. javanica previously disinfected, at room temperature (23-26oC) and with natural light. After 96 hours, it was counted in every dish the number of living, dead and parasitized nematodes, plus eggs parasitized or not parasitized. Subsequently, a logistic regression analysis (RL) for the evaluated variables was performed, which showed significant differences (p<0.0001) in the performance of the evaluated isolates, presenting ranges from 10% to 56% in dead nematodes, 13% to 79% in parasitic nemato- des and 1% to 96% in parasitized eggs. In general, isolates of Trichoderma sp. (Tri1, Tri2, Tri3 and Tri4) were the most efficient in the parasitism over juveniles and eggs of M. javanica. | |
dc.description.abstract | Se evaluó el parasitismo in vitro de 14 aislamientos de hongos nematófagos (6 Trichoderma sp., 4 Paecilomyces sp., 2 Fusarium oxysporum, y 2 Monacrosporium sp.) contra juveniles y huevos del nematodo agallador Meloidogyne javanica. Los hongos, de 3 regiones productoras de arroz en Costa Rica, (Pacífico Central, Huetar Atlántica y Chorotega), se aislaron mediante el método de espolvoreado en placas, durante abril del 2008 a agosto de 2009. Se utilizó un diseño completamente al azar con 10 repeticiones por hongo, en agar-agua (AA). Se inoculó una suspensión de 1 a 1,5x10 6 conidios (ufc.ml -1 ) por plato Petri, y al cuarto día se depositó en 0,5 ml una suspensión de 150 juveniles y 100 huevos de M. javanica previamente desinfectados, a temperatura ambiente (23-26°C) y con luz natural. Transcurridas 96 h se contó en cada plato el número de nematodos vivos, muertos ó parasi- tados y de huevos parasitados o no parasitados. Se realizó un análisis de regresión logística (RL) para las variables evaluadas, el cual mostró diferencias significativas (p<0,0001) en el desempeño de los hongos evaluados, presentando rangos de entre 10% a 56% en nematodos muertos, de 13% a 79% en nematodos parasitados y de 1% a 96% en huevos parasitados. En general, los hongos del género Trichoderma (Tri1, Tri2, Tri3 y Tri4) fueron los más eficientes en el parasitismo sobre juveniles y huevos de M. javanica. | |
dc.format.extent | - | |
dc.relation.ispartof | Agronomía Costarricense Vol. 0 Núm. 0 | |
dc.subject | Pruebas in vitro | |
dc.subject | hongos nematófagos | |
dc.subject | capacidad depredadora | |
dc.subject | Meloidogyne sp | |
dc.title | In vitro evaluation of nematophagous fungi of Costa Rica rice-growing regions against the root-knot Meloidogyne javanica | |
dc.title | Evaluación in vitro de hongos nematófagos en zonas arroceras de Costa Rica contra el nematodo agallador Meloidogyne javanica | |
dc.type | artículo original | |
dc.date.updated | 2015-06-09T22:41:58Z | |
dc.language.rfc3066 | es |