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Identification of residues critical for toxicity in Clostridium perfringens phospholipase C, the key toxin in gas gangrene

Alape Girón, Alberto; Flores Díaz, Marietta; Guillouard, Isabelle; Naylor, Claire E.; Titball, Richard; Rucavado Romero, Alexandra; Lomonte, Bruno; Basak, Ajit K.; Gutiérrez, José María; Cole, Steward T.; Thelestam, Mónica (2000-08)

Clostridium perfringens phospholipase C (PLC), also called α-toxin, is the major virulence factor in the pathogenesis of gas gangrene. The toxic activities of genetically engineered α-toxin variants harboring single ...

Isolation and characterization of four medium-size disintegrins from the venoms of Central American viperid snakes of the genera Atropoides, Bothrops, Cerrophidion and Crotalus

Angulo Ugalde, Yamileth; Castro Godínez, Adriana; Lomonte, Bruno; Rucavado Romero, Alexandra; Fernández Ulate, Julián; Calvete Chornet, Juan José; Gutiérrez, José María (2014-12)

Four disintegrins were isolated from the venoms of the Central American viperid snakes Atropoides mexicanus (atropoimin), Bothrops asper (bothrasperin), Cerrophidion sasai (sasaimin), and Crotalus simus (simusmin). ...

Understanding structural and functional aspects of PII snake venom metalloproteinases: Characterization of BlatH1, a hemorrhagic dimeric enzyme from the venom of Bothriechis lateralis

Camacho Umaña, Erika; Villalobos Chacón, Eva; Sanz, Libia; Pérez, Alicia; Escalante Muñoz, Teresa; Lomonte, Bruno; Calvete Chornet, Juan José; Gutiérrez, José María; Rucavado Romero, Alexandra (2014-06)

A new homodimeric PII metalloproteinase, named BlatH1, was purified from the venom of the Central American arboreal viperid snake Bothriechis lateralis by a combination of anion-exchange chromatography, hydrophobic interaction ...

Comparison of venom composition and biological activities of the subspecies Crotalus lepidus lepidus, Crotalus lepidus klauberi and Crotalus lepidus morulus from Mexico

Martínez Romero, Gerardo; Rucavado Romero, Alexandra; Lazcano, David; Gutiérrez, José María; Borja, Miguel; Lomonte, Bruno; Garza García, Yolanda; Zugasti Cruz, Alejandro (2013-08-01)

The rock rattlesnakes Crotalus lepidus comprise a group (lepidus, klauberi, morulus and maculosus) of poorly known mountain cold-tolerant snakes in Mexico. In particular, Crotalus lepidus morulus is a snake endemic of ...

Tissue pathology induced by snake venoms: How to understand a complex pattern of alterations from a systems biology perspective?

Gutiérrez, José María; Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Lomonte, Bruno; Angulo Ugalde, Yamileth; Fox, Jay W. (2010-01)

The study of snake venom-induced local tissue pathology has been predominantly based on experimental designs and concepts that view tissue alterations from a dichotomous, homogeneous and reductionist perspective. Experimental ...

Local tissue damage induced by BaP1, a metalloproteinase isolated from Bothrops asper (Terciopelo) snake venom

Rucavado Romero, Alexandra; Lomonte, Bruno; Ovadia, Michael; Gutiérrez, José María (1995-12)

The pathogenesis of hemorrhage and other local effects induced by the metalloproteinase BaP1, isolated from Bothrops asper venom, was investigated using various in vivo and in vitro models. Upon intramuscular injection in ...

Neutralization of local tissue damage induced by Bothrops asper (terciopelo) snake venom

Gutiérrez, José María; León Montero, Guillermo; Rojas Céspedes, Gustavo; Lomonte, Bruno; Rucavado Romero, Alexandra; Chaves Mora, Fernando (1998-11)

Local tissue damage represents a serious consequence of Bothrops asper envenomations. It encompasses a complex series of alterations, including myonecrosis, dermonecrosis, hemorrhage and edema. Due to its rapid development ...

Skeletal muscle necrosis and regeneration after injection of Thalassophryne nattereri (niquim) fish venom in mice

Lopes Ferreira, Mónica; Núñez, Javier; Rucavado Romero, Alexandra; Farsky, Sandra H. P.; Lomonte, Bruno; Angulo Ugalde, Yamileth; Moura Da Silva, Ana M.; Gutiérrez, José María (2001-02)

Stings by Thalassophryne nattereri are responsible for envenomation of fishermen in north-eastern Brazil. Its venom induces prominent local tissue damage, characterized by pain, oedema and necrosis. The pathogenesis of ...

Proteomic and functional profiling of the venom of Bothrops ayerbei from Cauca, Colombia, reveals striking interspecific variation with Bothrops asper venom

Mora Obando, Diana; Guerrero Vargas, Jimmy; Prieto Sánchez, Rodrigo; Beltrán, José; Rucavado Romero, Alexandra; Sasa Marín, Mahmood; Gutiérrez, José María; Ayerbe, Santiago; Lomonte, Bruno (Journal of Proteomics Volume 96, 16 January 2014, Pages 159–172, 2014-01)

Bothrops ayerbei, a pitviper inhabiting the Patía River's basin (Valle Alto del Río Patía) in the Southwestern Department of Cauca, Colombia, was considered as a variant form of Bothrops asper prior to being proposed as a ...

Snake Venomics of the Lesser Antillean Pit Vipers Bothrops caribbaeus and Bothrops lanceolatus: Correlation with Toxicological Activities and Immunoreactivity of a Heterologous Antivenom

Gutiérrez, José María; Sanz, Libia; Escolano, José; Fernández Ulate, Julián; Lomonte, Bruno; Angulo Ugalde, Yamileth; Rucavado Romero, Alexandra; Warrell, David A.; Calvete Chornet, Juan José (2008-10)

The venom proteomes of the snakes Bothrops caribbaeus and Bothrops lanceolatus, endemic to the Lesser Antillean islands of Saint Lucia and Martinique, respectively, were characterized by reversephase HPLC fractionation, ...