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A constant area monolayer method to assess optimal lipid packing for lipolysis tested with several secreted phospholipases A2 

Yunes Quartino, Pablo J.; Portela, Madelón; Lima, Analía; Durán, Rosario; Lomonte, Bruno; Fidelio, Gerardo Daniel (2015-10)
We present an analysis of lipid monolayer hydrolysis at a constant area to assess the optimal lateral surface pressure value (Πopt) and thus, the surface packing density of the lipid, at which the activity of a given ...

A catalytically-inactive snake venom Lys49 phospholipase A2 homolog induces expression of cyclooxygenase-2 and production of prostaglandins through selected signaling pathways in macrophages 

Moreira, Vanessa; de Castro Souto, Pollyana Cristina Maggio; Ramirez Vinolo, Marco Aurélio; Lomonte, Bruno; Gutiérrez, José María; Curi, Rui; Teixeira, Catarina de Fátima (2013-05-15)
The effects of a snakevenom Lys-49phospholipaseA2 (PLA2) homolog named MT-II,devoid of enzymatic activity, on the biosynthesis of prostaglandins and protein expression of cyclooxygenase-2(COX-2) and signaling pathways ...

Amino acid sequence and biological characterization of BlatPLA2, a non-toxic acidic phospholipase A2 from the venom of the arboreal snake Bothriechis lateralis from Costa Rica 

Van der Laat Villalobos, Marco; Fernández Ulate, Julián; Durban, Jordi; Villalobos Chacón, Eva; Camacho Umaña, Erika; Calvete Chornet, Juan José; Lomonte, Bruno (2013-10)
Bothriechis is considered a monophyletic, basal genus of arboreal Neotropical pitvipers distributed across Middle America. The four species found in Costa Rica (B. lateralis, B. schlegeli, B. nigroviridis, B. supraciliaris) ...
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Preclinical evaluation of the efficacy of antivenoms for snakebite envenoming: State-of-the-art and challenges ahead 

Solano Trejos, María Gabriela; Pla Ferrer, Davinia; Herrera Vega, María; Segura Ruiz, Álvaro; Vargas Arroyo, Mariángela; Villalta Arrieta, Mauren; Sánchez Brenes, Andrés; Sanz, Libia; Lomonte, Bruno; León Montero, Guillermo; Calvete Chornet, Juan José; Gutiérrez, José María (2017-05)
Animal-derived antivenoms constitute the mainstay in the therapy of snakebite envenoming. The efficacy of antivenoms to neutralize toxicity of medically-relevant snake venoms has to be demonstrated through meticulous ...

Immunochemical characterization and role in toxic activities of region 115-129 of myotoxin II, a Lys49 phospholipase A2 from Bothrops asper snake venom 

Calderón Fuentes, Leonel; Lomonte, Bruno (1998-10)
The region 115–129 of myotoxin II, a catalytically inactive Lys49 phospholipase A2, was previously shown to constitute a heparin-binding site and to be involved in its cytolytic action in vitro. An immunochemical ...

Bactericidal activity of Lys49 and Asp49 myotoxic phospholipases A2 from Bothrops asper snake venom: synthetic Lys49 myotoxin II-(115-129)-peptide identifies its bactericidal region 

Páramo Aguilera, Leandro Alberto; Lomonte, Bruno; Pizarro Cerdá, Javier; Bengoechea, José Antonio; Gorvel, Jean Pierre; Moreno Robles, Edgardo (1998)
Mammalian group-II phospholipases A2 (PLA2) of inflammatory fluids display bactericidal properties, which are dependent on their enzymatic activity. This study shows that myotoxins II (Lys49) and III (Asp49), two group-II ...

Identification of the myotoxic site of the Lys49 phospholipase A2 from Agkistrodon piscivorus piscivorus snake venom: synthetic C-terminal peptides from Lys49, but not from Asp49 myotoxins, exert membrane-damaging activities 

Núñez, Carlos E.; Angulo Ugalde, Yamileth; Lomonte, Bruno (2001)
Group II phospholipase A2 (PLA2) myotoxins found in the venoms of Crotalidae snakes can be divided into `Asp49' and `Lys49' isoforms, the latter being considered catalytically-inactive variants. Previous studies on one ...

Isolation and characterization of myotoxin II from Atropoides (Bothrops) nummifer snake venom, a new Lys49 phospholipase A2 homologu 

Angulo Ugalde, Yamileth; Olamendi Portugal, Timoteo; Possani, Lourival D.; Lomonte, Bruno (2000)
Myotoxic phospholipases A2 of class II are commonly found in the venoms of crotalid snakes. As an approach to understanding their structure±activity relationship, diverse natural variants have been characterized ...

Characterization of a basic phospholipase A2-homologue myotoxin isolated from the venom of the snake Bothrops neuwiedii (yarará chica) from Argentina 

Geoghegan, Patricia; Angulo Ugalde, Yamileth; Cangelosi, Adriana; Díaz, Mónica; Lomonte, Bruno (1999)
A basic protein was isolated by CM-Sephadex C-25 chromatography from the venom of Bothrops neuwiedii from Argentina, and named B. neuwiedii myotoxin I. This protein exerted local myotoxic and edema-forming e ects in mice, ...
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Bases moleculares del reconocimiento de antígenos 

Lomonte, Bruno (1999)
Aunque todos los organismos pertenecientes al reino animal poseen una serie de mecanismos inmunitarios que persiguen mantener su integridad y rechazar la invasión de material foráneo, solo los vertebrados cuentan con un ...
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Lomonte, Bruno (260)
Gutiérrez, José María (164)Angulo Ugalde, Yamileth (58)Calvete Chornet, Juan José (38)Sanz, Libia (30)Fernández Ulate, Julián (21)Rucavado, Alexandra (20)León Montero, Guillermo (16)Cerdas Fallas, Luis (15)Rojas Céspedes, Gustavo (15)... View MoreType
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  • Repositorios universitarios

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Repositorio Institucional de la Universidad de Costa Rica. Algunos derechos reservados. Este repositorio funciona con DSpace.
Universidad de Costa Rica