Search

Now showing items 1-10 of 19

Development of a chicken-derived antivenom against the taipan snake (Oxyuranus scutellatus) venom and comparison with an equine antivenom

Navarro Arias, Diego; Vargas Arroyo, Mariángela; Herrera Vega, María; Segura Ruiz, Álvaro; Gómez Argüello, Aarón; Villalta Arrieta, Mauren; Ramírez Arguedas, Nils Antonio; Williams, David J.; Gutiérrez, José María; León Montero, Guillermo (2016-09-15)

A chicken-derived antivenom (ChDAv) towards taipan snake (Oxyuranus scutellatus) venom was produced by purifying anti-taipan IgY from egg yolks of hens immunized with taipan venom. The productivity, antivenomic profile, ...

Effect of geographical variation of Echis ocellatus, Naja nigricollis and Bitis arietans venoms on their neutralization by homologous and heterologous antivenoms

Sánchez Brenes, Andrés; Coto Porras, Jorge; Segura Ruiz, Álvaro; Vargas Arroyo, Mariángela; Solano Trejos, María Gabriela; Herrera Vega, María; Villalta Arrieta, Mauren; Estrada Umaña, Ricardo; Gutiérrez, José María (2015-12-15)

Two antivenoms prepared by using Echis ocellatus, Bitis arietans and Naja nigricollis venoms from different locations in sub-Saharan Africa were compared for their neutralizing ability. Both antivenoms were similarly ...

Purification of equine whole IgG snake antivenom by using an aqueous two phase system as a primary purification step

Vargas Arroyo, Mariángela; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Herrera Vega, María; Gutiérrez, José María; León Montero, Guillermo (2015-01)

There is a need to introduce innovations in the manufacture of snake antivenoms to increase the supply of these products worldwide. In this work, the fractionation of equine hyperimmune plasma with a new methodology that ...

Neutralization of the neuromuscular inhibition of venom and taipoxin from the taipan (Oxyuranus scutellatus) by F(ab0)2 and whole IgG antivenoms

Herrera Vega, María; de Cássia de O. Collaço, Rita; Villalta Arrieta, Mauren; Segura Ruiz, Álvaro; Vargas Arroyo, Mariángela; Wright, Christine E.; Paiva, Owen K.; Matainaho, Teatulohi; Jensen, Simon D.; León Montero, Guillermo; Williams, David J.; Rodrigues Simioni, Lea; Gutiérrez, José María (2016-01-22)

The neuromuscular junction activity of Oxyuranus scutellatus venom and its presynaptic neurotoxin, taipoxin, and their neutralization by two antivenoms were examined in mouse phrenic nerve-diaphragm preparations. The action ...

Antivenomic Characterization of Two Antivenoms Against the Venom of the Taipan, Oxyuranus scutellatus, from Papua New Guinea and Australia

Herrera Vega, María; Paiva, Owen K.; Pagotto, Ana Helena; Segura Ruiz, Álvaro; Serrano, Solange M. T.; Vargas Arroyo, Mariángela; Villalta Arrieta, Mauren; Jensen, Simon D.; León Montero, Guillermo; Williams, David J.; Gutiérrez, José María (2014-11-05)

Antivenoms manufactured by bioCSL Limited (Australia) and Instituto Clodomiro Picado (Costa Rica) against the venom of the taipan snakes (Oxyuranus scutellatus) from Australia and Papua New Guinea (PNG), respectively, were ...

Freeze-dried snake antivenoms formulated with sorbitol, sucrose or mannitol: Comparison of their stability in an accelerated test

Herrera Vega, María; Tattini Jr., Virgilio; Pitombo, Ronaldo N. M.; Gutiérrez, José María; Borgognoni, Camila; Vega Baudrit, José Roberto; Solera, Federico; Cerdas Solís, Maykel; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; León Montero, Guillermo (2014-11)

Freeze-drying is used to improve the long term stability of pharmaceutical proteins. Sugars and polyols have been successfully used in the stabilization of proteins. However, their use in the development of freeze-dried ...

Toxicity of Bothrops sp snake venoms from Ecuador and preclinical assessment of the neutralizing efficacy of a polyspecific antivenom from Costa Rica

Laines, Johana; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Herrera Vega, María; Vargas Arroyo, Mariángela; Alvarez, Gladys; Gutiérrez, José María; León Montero, Guillermo (2014-09-15)

The toxicological profile of the venoms of the snakes Bothrops asper and Bothrops atrox from Ecuador was investigated, together with the venom of a population of B. asper formerly classified as ‘Bothrops xanthogrammus’. ...

Pathogenic mechanisms underlying adverse reactions induced by intravenous administration of snake antivenoms

León Montero, Guillermo; Herrera Vega, María; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Gutiérrez, José María (2013-12-15)

Snake antivenoms are formulations of immunoglobulins, or immunoglobulin fragments, purified from the plasma of animals immunized with snake venoms. Their therapeutic success lies in their ability to mitigate the progress ...

Assessing the preclinical efficacy of antivenoms: From the lethality neutralization assay to antivenomics

Gutiérrez, José María; Solano Trejos, María Gabriela; Pla Ferrer, Davinia; Herrera Vega, María; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Sanz, Libia; Lomonte, Bruno; Calvete Chornet, Juan José; León Montero, Guillermo (2013-07)

The assessment of the capacity of antivenoms to neutralize the lethal activity of snake venoms is the gold standard in the preclinical analysis of antivenom efficacy, and is routinely performed by manufacturers and quality ...

Comparison of the adjuvant activity of aluminum hydroxide and calcium phosphate on the antibody response towards Bothrops asper snake venom

Olmedo, Hidekel; Herrera Vega, María; Rojas Sánchez, Leonardo; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Leiguez, Elbio; Teixeira, Catarina de Fátima; Estrada Umaña, Ricardo; Gutiérrez, José María; León Montero, Guillermo; Montero Villalobos, Mavis Lili (2014)

The adjuvanticity of aluminum hydroxide and calcium phosphate on the antibody response in mice towards the venom of the snake Bothrops asper was studied. It was found that, in vitro, most of the venom proteins are similarly ...