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Proteomic analysis of Bothrops pirajai snake venom and characterization of BpirMP, a new P-I metalloproteinase

Bernardes, Carolina P.; Menaldo, Danilo L.; Camacho Umaña, Erika; Rosa, José C.; Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Lomonte, Bruno; Gutiérrez, José María; Sampaio, Suely V.; Rosa, José C. (2013-03-27)

Bothrops pirajai snake venom was analyzed by a proteomic strategy. Proteins were separated by RP-HPLC, followed by SDS-PAGE, in-gel tryptic digestion, identification by MALDI-TOF/TOF mass spectrometry, and assignment to ...

Homogenates of skeletal muscle injected with snake venom inhibit myogenic differentiation in cell culture

Saravia Otten, Patricia; Robledo, Bárbara; Escalante Muñoz, Teresa; Bonilla, Llira; Rucavado Romero, Alexandra; Lomonte, Bruno; Hernández, Rosario; Flock, Jan-Ingmar; Gutiérrez, José María; Gastaldello, Stefano (2013-02)

Introduction: Viperid snakebite envenomings are characterized by muscle necrosis and a deficient regenerative response. Methods: Homogenates from gastrocnemius muscles of mice injected with the venom of the snake Bothrops ...

Tissue pathology induced by snake venoms: How to understand a complex pattern of alterations from a systems biology perspective?

Gutiérrez, José María; Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Lomonte, Bruno; Angulo Ugalde, Yamileth; Fox, Jay W. (2010-01)

The study of snake venom-induced local tissue pathology has been predominantly based on experimental designs and concepts that view tissue alterations from a dichotomous, homogeneous and reductionist perspective. Experimental ...

Unresolved issues in the understanding of the pathogenesis of local tissue damage induced by snake venoms

Gutiérrez, José María; Rucavado Romero, Alexandra; Escalante Muñoz, Teresa; Herrera Arias, Cristina; Fernández Ulate, Julián; Lomonte, Bruno; Fox, Jay W. (2018-06)

Snakebite envenoming by viperid species, and by some elapids, is characterized by a complex pattern of tissue damage at the anatomical site of venom injection. In severe cases, tissue destruction may be so extensive as ...

Novel catalytically-inactive PII metalloproteinases from a viperid snake venom with substitutions in the canonical zinc-binding motif

Camacho Umaña, Erika; Sanz, Libia; Escalante Muñoz, Teresa; Pérez, Alicia; Villalta Romero, Fabián; Lomonte, Bruno; Neves Ferreira, Ana Gisele da Costa; Feoli Grant, Andrés; Calvete Chornet, Juan José; Gutiérrez, José María; Rucavado Romero, Alexandra (2016-10-12)

Snake venom metalloproteinases (SVMPs) play key biological roles in prey immobilization and digestion. The majority of these activities depend on the hydrolysis of relevant protein substrates in the tissues. Hereby, we ...

Understanding structural and functional aspects of PII snake venom metalloproteinases: Characterization of BlatH1, a hemorrhagic dimeric enzyme from the venom of Bothriechis lateralis

Camacho Umaña, Erika; Villalobos Chacón, Eva; Sanz, Libia; Pérez, Alicia; Escalante Muñoz, Teresa; Lomonte, Bruno; Calvete Chornet, Juan José; Gutiérrez, José María; Rucavado Romero, Alexandra (2014-06)

A new homodimeric PII metalloproteinase, named BlatH1, was purified from the venom of the Central American arboreal viperid snake Bothriechis lateralis by a combination of anion-exchange chromatography, hydrophobic interaction ...