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Isolation and characterization of a myotoxic phospholipase A2 from the venom of the arboreal snake Bothriechis (Bothrops) schlegelii from Costa Rica

Angulo Ugalde, Yamileth; Chaves Olarte, Esteban; Rucavado Romero, Alexandra; Gutiérrez, José María; Lomonte, Bruno; Alape Girón, Alberto (1997-03)

A new myotoxic phospholipase A2was isolated from the venom of the arboreal snakeBothriechis schlegelii(formerlyBothrops schlegelii) from Costa Rica, by ion-exchange chromatography on CM-Sephadex.B. schlegeliimyotoxin I is ...

Neutralization of Bothrops asper venom by antibodies, natural products, and synthetic drugs: contributions to understanding snakebite envenomings and their treatment

Lomonte, Bruno; León Montero, Guillermo; Angulo Ugalde, Yamileth; Rucavado Romero, Alexandra; Núñez Rangel, Vitelbina (2009)

Interest in studies on the neutralization of snake venoms and toxins by diverse types of inhibitors is two-fold. From an applied perspective, results enclose the potential to be translated into useful therapeutic products ...

Local tissue damage induced by BaP1, a metalloproteinase isolated from Bothrops asper (Terciopelo) snake venom

Rucavado Romero, Alexandra; Lomonte, Bruno; Ovadia, Michael; Gutiérrez, José María (1995-12)

The pathogenesis of hemorrhage and other local effects induced by the metalloproteinase BaP1, isolated from Bothrops asper venom, was investigated using various in vivo and in vitro models. Upon intramuscular injection in ...

Proteomic analysis of Bothrops pirajai snake venom and characterization of BpirMP, a new P-I metalloproteinase

Bernardes, Carolina P.; Menaldo, Danilo L.; Camacho Umaña, Erika; Rosa, José C.; Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Lomonte, Bruno; Gutiérrez, José María; Sampaio, Suely V.; Rosa, José C. (2013-03-27)

Bothrops pirajai snake venom was analyzed by a proteomic strategy. Proteins were separated by RP-HPLC, followed by SDS-PAGE, in-gel tryptic digestion, identification by MALDI-TOF/TOF mass spectrometry, and assignment to ...

Homogenates of skeletal muscle injected with snake venom inhibit myogenic differentiation in cell culture

Saravia Otten, Patricia; Robledo, Bárbara; Escalante Muñoz, Teresa; Bonilla, Llira; Rucavado Romero, Alexandra; Lomonte, Bruno; Hernández, Rosario; Flock, Jan-Ingmar; Gutiérrez, José María; Gastaldello, Stefano (2013-02)

Introduction: Viperid snakebite envenomings are characterized by muscle necrosis and a deficient regenerative response. Methods: Homogenates from gastrocnemius muscles of mice injected with the venom of the snake Bothrops ...

Anti-human erythrocyte antibodies in horse-derived antivenoms used in the treatment of snakebite envenomations

León Montero, Guillermo; Rodríguez, Miguel Angel; Rucavado Romero, Alexandra; Lomonte, Bruno; Gutiérrez, José María (2007-03)

This work examined the presence of antibodies reacting with human erythrocytes in horse-derived antivenoms used in the treatment of snakebite envenomations, and assessed the efficacy of various fractionation protocols in ...

Isolation and biochemical, functional and structural characterization of a novel L-amino acid oxidase from Lachesis muta snake venom

Bregge Silva, Cristiane; Nonato, Maria Cristina; de Albuquerque, Sérgio; Ho, Paulo Lee; Junqueira de Azevedo, Inácio de Loiola Meirelles; Vasconcelos Diniz, Marcelo Ribeiro; Lomonte, Bruno; Rucavado Romero, Alexandra; Díaz Oreiro, Cecilia; Gutiérrez, José María; Arantes, Eliane Candiani (2012-12-01)

The aim of this study was the isolation of the LAAO from Lachesis muta venom (LmLAAO) and its biochemical, functional and structural characterization. Two different purification protocols were developed and both provided ...

Comparison of biochemical and cytotoxic activities of extracts obtained from dorsal spines and caudal fin of adult and juvenile non-native Caribbean lionfish (Pterois volitans/miles)

Ortiz Chaves, Natalia; Lomonte, Bruno; Rucavado Romero, Alexandra; Díaz Oreiro, Cecilia; Sáenz Espinoza, Aránzazu (2017-10)

Pterois volitans/miles lionfish (adult and juvenile) dorsal spines and caudal fin extracts were compared in their general composition, enzymatic activities and hemolytic and cytotoxic effects on bovine aortic endothelial ...

Novel catalytically-inactive PII metalloproteinases from a viperid snake venom with substitutions in the canonical zinc-binding motif

Camacho Umaña, Erika; Sanz, Libia; Escalante Muñoz, Teresa; Pérez, Alicia; Villalta Romero, Fabián; Lomonte, Bruno; Neves Ferreira, Ana Gisele da Costa; Feoli Grant, Andrés; Calvete Chornet, Juan José; Gutiérrez, José María; Rucavado Romero, Alexandra (2016-10-12)

Snake venom metalloproteinases (SVMPs) play key biological roles in prey immobilization and digestion. The majority of these activities depend on the hydrolysis of relevant protein substrates in the tissues. Hereby, we ...

Comparison of venom composition and biological activities of the subspecies Crotalus lepidus lepidus, Crotalus lepidus klauberi and Crotalus lepidus morulus from Mexico

Martínez Romero, Gerardo; Rucavado Romero, Alexandra; Lazcano, David; Gutiérrez, José María; Borja, Miguel; Lomonte, Bruno; Garza García, Yolanda; Zugasti Cruz, Alejandro (2013-08-01)

The rock rattlesnakes Crotalus lepidus comprise a group (lepidus, klauberi, morulus and maculosus) of poorly known mountain cold-tolerant snakes in Mexico. In particular, Crotalus lepidus morulus is a snake endemic of ...