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Métodos de Investigación: destrezas básicas para el trabajo científico en las disciplinas biomédicas
(2020-10-01)
Esta obra didáctica nace con el objetivo de presentar a sus lectores una visión
introductoria sobre el fenómeno científico actual, sus orígenes, su contexto
costarricense, y posteriormente presentar las principales ...
Cytotoxicity of snake venom Lys49 PLA2-like myotoxin on rat cardiomyocytes ex vivo does not involve a direct action on the contractile apparatus
(2021-09)
Viperid snake venoms contain a unique family of cytotoxic proteins, the Lys49 PLA2 homologs, which are devoid of enzymatic activity but disrupt the integrity of cell membranes. They are known to induce skeletal muscle ...
Stable production of recombinant SARS-CoV-2 receptor-binding domain in mammalian cells with co-expression of a fluorescent reporter and its validation as antigenic target for COVID-19 serology testing
(2023-01)
SARS-CoV-2 receptor binding domain (RBD) recognizes the angiotensin converting enzyme 2 (ACE2) receptor in host cells that enables infection. Due to its antigenic specificity, RBD production is important for development ...
Venom of the neotropical rattlesnake, Crotalus culminatus: Intraspecific variation, neutralization by antivenoms, and immunogenicity in rabbits
(2024)
Crotalus culminatus is a medically significant species of rattlesnake in Mexico [1]. While the proteomic composition of its venom has been previously reported for both juvenile and adult specimens, there has been limited ...
Pros and cons of different therapeutic antibody formats for recombinant antivenom development
(2018-05)
Antibody technologies are being increasingly applied in the field of toxinology. Fuelled by the many
advances in immunology, synthetic biology, and antibody research, different approaches and antibody
formats are being ...
Characterization of Extracellular Vesicles Secreted by a Clinical Isolate of Naegleria fowleri and Identification of Immunogenic Components within Their Protein Cargo
(2022-06)
Abstract: Extracellular vesicles (EVs) are small lipid vesicles released by both prokaryotic and eu-karyotic cells, involved in intercellular communication, immunomodulation and pathogenesis. In this study, we performed a ...
Antivenomics: a proteomics tool for studying the immunoreactivity of antivenoms
(2015-03)
Envenoming following snakebite is largely a neglected threat to public health in tropical and subtropical regions of Africa, Asia, Latin America and Oceania addicting some of the world’s poorest rural communities. An ...
Novel catalytically-inactive PII metalloproteinases from a viperid snake venom with substitutions in the canonical zinc-binding motif
(2016-10-12)
Snake venom metalloproteinases (SVMPs) play key biological roles in prey immobilization
and digestion. The majority of these activities depend on the hydrolysis of relevant protein substrates
in the tissues. Hereby, we ...
Proteogenomic analysis of the Clostridium difficile exoproteome reveals a correlation between phylogenetic distribution and virulence potential
(2020)
C. difficile induces antibiotic-associated diarrhea due to the action of two secreted toxins, TcdA and TcdB. A considerable range of virulence among C. difficile strains has been widely reported. During a hospital outbreak, ...
A myotoxic Lys49 phospholipase A2-homologue is the major component of the venom of Bothrops cotiara from Misiones, Argentina
(2018-04-27)
Bothrops cotiara is a pitviper found in Southeastern Brazil and, scarcely, in the Misiones province of Argentina. In contrast to considerable information available on the venom of the Brazilian snake population, that of ...