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Snake Venomics of the Central American Rattlesnake Crotalus simus and the South American Crotalus durissus Complex Points to Neurotoxicity as an Adaptive Paedomorphic Trend along Crotalus Dispersal in South America

Calvete Chornet, Juan José; Sanz, Libia; Cid, Pedro; De La Torre, Pilar; Flores Díaz, Marietta; Dos Santos, María Cristina; Borges, Adolfo; Bremo, Adolfo; Angulo Ugalde, Yamileth; Lomonte, Bruno; Alape Girón, Alberto; Gutiérrez, José María (2010)

We report a comparative venomic and antivenomic characterization of the venoms of newborn and adult specimens of the Central American rattlesnake, Crotalus simus, and of the subspecies cumanensis, durissus, ruruima, and ...

Integrated “omics” profiling indicates that miRNAs are modulators of the ontogenetic venom composition shift in the Central American rattlesnake, Crotalus simus simus

Durban, Jordi; Pérez, Alicia; Sanz, Libia; Gómez Argüello, Aarón; Bonilla Murillo, Fabián; Rodríguez Arguedas, Santos; Chacón Gutiérrez, Danilo; Sasa Marín, Mahmood; Angulo Ugalde, Yamileth; Gutiérrez, José María; Calvete Chornet, Juan José (2013)

Background Understanding the processes that drive the evolution of snake venom is a topic of great research interest in molecular and evolutionary toxinology. Recent studies suggest that ontogenetic changes in venom ...

Preclinical assessment of a polyspecific antivenom against the venoms of Cerrophidion sasai, Porthidium nasutum and Porthidium ophryomegas: Insights from combined antivenomics and neutralization assays

Gutiérrez, José María; Tsai, Wan-Chih; Pla Ferrer, Davinia; Solano Trejos, María Gabriela; Lomonte, Bruno; Sanz, Libia; Angulo Ugalde, Yamileth; Calvete Chornet, Juan José (2013-03-15)

A polyspecific antivenom is used in Central America for the treatment of envenomings by viperid snakes. This antivenom is generated in horses hyperimmunized with a mixture of venoms from Bothrops asper, Crotalus simus and ...

Venomic and antivenomic analyses of the Central American coral snake, Micrurus nigrocinctus (Elapidae)

Fernández Ulate, Julián; Alape Girón, Alberto; Angulo Ugalde, Yamileth; Sanz, Libia; Gutiérrez, José María; Calvete Chornet, Juan José; Lomonte, Bruno (2011-01-31)

The proteome of the venom of Micrurus nigrocinctus (Central American coral snake) was analyzed by a “venomics” approach. Nearly 50 venom peaks were resolved by RP-HPLC, revealing a complex protein composition. Comparative ...

Snake population venomics and antivenomics of Bothrops atrox: Paedomorphism along its transamazonian dispersal and implications of geographic venom variability on snakebite management

Calvete Chornet, Juan José; Sanz, Libia; Pérez, Alicia; Borges, Adolfo; Vargas, Alba M.; Lomonte, Bruno; Angulo Ugalde, Yamileth; Gutiérrez, José María; Chalkidis, Hipócrates M.; Mourão, Rosa H.V.; Furtado, María de Fátima; Moura Da Silva, Ana M. (2011-04-11)

We describe two geographically differentiated venom phenotypes across the wide distribution range of Bothrops atrox, from the Colombian Magdalena Medio Valley through Puerto Ayacucho and El Paují, in the Venezuelan States ...

Clinical and laboratory alterations in horses during immunization with snake venoms for the production of polyvalent (Crotalinae) antivenom

Angulo Ugalde, Yamileth; Estrada Umaña, Ricardo; Gutiérrez, José María (1997-01)

Six horses were immunized with the venoms of Bothrops asper, Crotalus durissus durissus and Lachesis muta stenophrys for the production of polyvalent (Crotalinae) antivenom. During the immunization, clinical and laboratory ...

Snake venomics and antivenomics of Protobothrops mucrosquamatus and Viridovipera stejnegeri from Taiwan: Keys to understand the variable immune response in horses

Villalta Arrieta, Mauren; Pla Ferrer, Davinia; Yang, Su Lin; Sanz, Libia; Segura Ruiz, Álvaro; Vargas Arroyo, Mariángela; Chen, Pei Yu; Herrera Vega, María; Estrada Umaña, Ricardo; Cheng, Ya Fen; Lee, Cheng Dow; Cerdas Solís, Maykel; Chiang, Jen Ron; Angulo Ugalde, Yamileth; León Montero, Guillermo; Calvete Chornet, Juan José; Gutiérrez, José María (2012-10-22)

The proteomes of the venoms of the snakes Viridovipera stejnegeri and Protobothrops mucrosquamatus from Taiwan were characterized by N-terminal sequencing, MALDI-TOF mass fingerprinting, and collision-induced dissociation ...

Screening for target toxins of the antiophidic protein DM64 through a gel-based interactomics approach

Rocha, Surza Lucia Gonçalves; Neves Ferreira, Ana Gisele da Costa; Trugilho, Monique Ramos de Oliveira; Angulo Ugalde, Yamileth; Lomonte, Bruno; Valente, Richard H.; Domont, Gilberto B.; Perales, Jonas (2017-01)

DM64 is a glycosylated protein with antivenomactivity isolated fromthe serum of the opossumDidelphis aurita. It binds non-covalently to myotoxins I (Asp49) and II (Lys49) from Bothrops asper venom and inhibits their myotoxic ...

Antimicrobial activity of myotoxic phospholipases A2 from crotalid snake venoms and synthetic peptide variants derived from their C-terminal region

Santamaría Quesada, Carlos Manuel; Larios Ramos, Silda; Angulo Ugalde, Yamileth; Pizarro Cerdá, Javier; Gorvel, Jean Pierre; Moreno Robles, Edgardo; Lomonte, Bruno (2005)

A short peptide derived from the C-terminal region of Bothrops asper myotoxin II, a Lys49 phospholipase A2 (PLA2), was previously found to reproduce the bactericidal activity of its parent molecule. In this study, a panel ...

Structure of myotoxin-II, a catalytically inactive Lys49 phospholipase A2 homologue from Atropoides nummifer venom

Murakami, Mário T.; Melo, Cristiane C.; Angulo Ugalde, Yamileth; Lomonte, Bruno; Arni, Raghuvir K. (2006)

Lys49 snake-venom phospholipase A2 (PLA2) homologues are highly myotoxic proteins which, although lacking catalytic activity, possess the ability to disrupt biological membranes, inducing significant muscle-tissue loss ...