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Electrophoretic and immunochemical studies of Micrurus venoms
(1994)
The electrophoretic mobilities of venom components from 15 Micrurus species were studied by polyacrylamide gel electrophoresis. The venoms showed species-specific protein patterns under native (PAGE) or denaturing (urea-PAGE) ...
Venomic and antivenomic analyses of the Central American coral snake, Micrurus nigrocinctus (Elapidae)
(2011-01-31)
The proteome of the venom of Micrurus nigrocinctus (Central American coral snake) was analyzed by a “venomics” approach. Nearly 50 venom peaks were resolved by RP-HPLC, revealing a complex protein composition. Comparative ...
Lemnitoxin, the major component of Micrurus lemniscatus coral snake venom, is a myotoxic and pro-inflammatory phospholipase A2
(2016-08-26)
The venom of Micrurus lemniscatus, a coral snake of wide geographical distribution in South America, was fractionated by reverse-phase HPLC and the fractions screened for phospholipase A2 (PLA2) activity. The major component ...
Immunochemical characterization of Micrurus nigrocinctus nigrocinctus venom with monoclonal and polyclonal antibodies
(1994)
Eleven murine monocloncal antibodies (MAbs) against Micrurus nigrocinctus nigrocinctus venom were produced and partially characterized. When M. n. nigrocinctus venom proteins were separated by SDS-PAGE under non-reducing ...
Snake Venomics of the Central American Rattlesnake Crotalus simus and the South American Crotalus durissus Complex Points to Neurotoxicity as an Adaptive Paedomorphic Trend along Crotalus Dispersal in South America
(2010)
We report a comparative venomic and antivenomic characterization of the venoms of newborn and adult specimens of the Central American rattlesnake, Crotalus simus, and of the subspecies cumanensis, durissus, ruruima, and ...
Development of immunoassays for determination of circulating venom antigens during envenomations by coral snakes (Micrurus species)
(1997-11)
A reverse agglutination assay and two capture enzyme-linked immunoassays (ELISAs) for the quantitative determination of Micrurus nigrocinctus nigrocinctus venom antigens in fluids were developed using affinity-purified ...
Cleavage of the NH2-Terminal Octapeptide of Bothrops asper Myotoxic Lysine-49 Phospholipase A2 Reduces Its Membrane-Destabilizing Effect
(1994-08)
Bothrops asper myotoxin II was cleaved with cyanogen bromide to determine the role of NH2-terminal amino acid residues in its ability to destabilize negatively charged liposomes and to induce myonecrosis. After treatment, ...
Isolation and characterization of a myotoxic phospholipase A2 from the venom of the arboreal snake Bothriechis (Bothrops) schlegelii from Costa Rica
(1997-03)
A new myotoxic phospholipase A2was isolated from the venom of the arboreal snakeBothriechis schlegelii(formerlyBothrops schlegelii) from Costa Rica, by ion-exchange chromatography on CM-Sephadex.B. schlegeliimyotoxin I is ...
Structural characterization and phylogenetic relationships of myotoxin II from Atropoides (Bothrops) nummifer snake venom, a Lys49 phospholipase A2 homologue
(2002)
In order to analyze its structure–function relationships, the complete amino acid sequence of myotoxin II from Atropoides
(Bothrops) nummifer from Costa Rica was determined. This toxin is a Lys49-type phospholipase A2 ...
Identification of residues critical for toxicity in Clostridium perfringens phospholipase C, the key toxin in gas gangrene
(2000-08)
Clostridium perfringens phospholipase C (PLC), also called α-toxin, is the major virulence factor in the pathogenesis of gas gangrene. The toxic activities of genetically engineered α-toxin variants harboring single ...