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dc.contributor.authorMora Rodríguez, Rodrigo
dc.contributor.authorSiles Canales, Francisco
dc.contributor.authorCastro, Jorge
dc.contributor.authorSáenz Espinoza, Aránzazu
dc.contributor.authorCalderón Ramírez, Saúl
dc.date.accessioned2015-09-07T16:41:22Z
dc.date.available2015-09-07T16:41:22Z
dc.date.issued2014-10-29
dc.identifier.citationhttp://link.springer.com/chapter/10.1007/978-3-319-13117-7_89#page-1es_ES
dc.identifier.urihttp://hdl.handle.net/10669/15251
dc.descriptionartículo -- Universidad de Costa Rica, Escuela de Ingeniería Eléctrica. 2014es_ES
dc.description.abstractIn this work we implement an automatic videobased online cell tracking system for TNFa treated cells in the transcription factor NF-kB pathway, with the aim to provide p65 measurement approximation with time and individual cell resolution. The video used to test our proposed tracking system consists on cells transfected with fluorescent p65 protein. Such protein switches its location within the cell, among the nucleus and cytoplasm over time, causing important changes on its apparent morphology. This represents a major challenge for an automatic cell tracking system. Additionally, images in the tested microscopy footage present noise and contrast variations. For noise reduction, we modified the deceived bilateral filter (DBF), a denoising and edge sharpening non linear filter. The proposed modification of the DBF consists in an adaptive border enhancement. We tested the cell tracking algorithm with different preprocessing configurations achieving a maximum tracking accuracy improvement of 28%, with the use of the modified DBFes_ES
dc.description.sponsorshipUniversidad de Costa Rica, Sistema de Estudios de Posgrado (SEP)es_ES
dc.language.isoen_USes_ES
dc.relation.ispartofseriesIFMBE Proceedings
dc.sourceVI Latin American Congress on Biomedical Engineering CLAIB 2014, Paraná, Argentina 29, 30 & 31 October 2014 Volume 49 of the series IFMBE Proceedings pp 345-348es_ES
dc.subjectnline trackinges_ES
dc.subjectautomatic cell activity analysises_ES
dc.subjectNF-kB pathwayes_ES
dc.subjecttranscription factorses_ES
dc.subjectDBFes_ES
dc.titleDeceived bilateral filter for improving the automatic cell segmentation and tracking in the NF-kB pathway without nuclear staininges_ES
dc.typeinfo:eu-repo/semantics/preprintes_ES
dc.typePreprintes_ES
dc.identifier.doi0.1007/978-3-319-13117-7_89
dc.description.procedenceUCR::Docencia::Ingeniería::Facultad de Ingeniería::Escuela de Ingeniería Eléctricaes_ES


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