Listar Microbiología por autor "f998c9d9-0307-4443-8ce8-92b217ff60e9"
Mostrando ítems 1-9 de 9
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Amino acid sequence and biological characterization of BlatPLA2, a non-toxic acidic phospholipase A2 from the venom of the arboreal snake Bothriechis lateralis from Costa Rica
Van der Laat Villalobos, Marco; Fernández Ulate, Julián; Durban, Jordi; Villalobos Chacón, Eva; Camacho Umaña, Erika; Calvete Chornet, Juan José; Lomonte, Bruno (2013-10)Bothriechis is considered a monophyletic, basal genus of arboreal Neotropical pitvipers distributed across Middle America. The four species found in Costa Rica (B. lateralis, B. schlegeli, B. nigroviridis, B. supraciliaris) ... -
Novel catalytically-inactive PII metalloproteinases from a viperid snake venom with substitutions in the canonical zinc-binding motif
Camacho Umaña, Erika; Sanz, Libia; Escalante Muñoz, Teresa; Pérez, Alicia; Villalta Romero, Fabián; Lomonte, Bruno; Neves Ferreira, Ana Gisele da Costa; Feoli Grant, Andrés; Calvete Chornet, Juan José; Gutiérrez, José María; Rucavado Romero, Alexandra (2016-10-12)Snake venom metalloproteinases (SVMPs) play key biological roles in prey immobilization and digestion. The majority of these activities depend on the hydrolysis of relevant protein substrates in the tissues. Hereby, we ... -
Proteomic analysis of Bothrops pirajai snake venom and characterization of BpirMP, a new P-I metalloproteinase
Bernardes, Carolina P.; Menaldo, Danilo L.; Camacho Umaña, Erika; Rosa, José C.; Escalante Muñoz, Teresa; Rucavado Romero, Alexandra; Lomonte, Bruno; Gutiérrez, José María; Sampaio, Suely Vilela; Rosa, José C. (2013-03-27)Bothrops pirajai snake venom was analyzed by a proteomic strategy. Proteins were separated by RP-HPLC, followed by SDS-PAGE, in-gel tryptic digestion, identification by MALDI-TOF/TOF mass spectrometry, and assignment to ... -
Proteomic and functional analysis of the venom of Porthidium lansbergii lansbergii (Lansberg’s hognose viper) from the Atlantic Department of Colombia
Jiménez Charris, Eliécer; Montealegre Sánchez, Leonel; Solano Redondo, Luis; Mora Obando, Diana; Camacho Umaña, Erika; Castro Herrera, Fernando; Fierro Pérez, Leonardo; Lomonte, Bruno (2015)The venom of the Lansberg's hognose pitviper, Porthidium lansbergii lansbergii, a species found in the northern region of Colombia, is poorly known. Aiming to increase knowledge on Porthidium species venoms, its proteomic ... -
Snake venomics, experimental toxic activities and clinical characteristics of human envenomation by Bothrocophias myersi (Serpentes: Viperidae) from Colombia
Pereañez, Jaime Andrés; Preciado Rojo, Lina María; Fernández Ulate, Julián; Camacho Umaña, Erika; Lomonte, Bruno; Castro Herrera, Fernando; Cañas Dávila, Carlos Alberto; Galvis Rizo, Carlos Andrés; Castaño Valencia, Rafael Santiago (2020)Venoms of the viperid genus Bothrocophias, restricted to Colombia and Ecuador, are poorly known. Only a proteomic analysis of B. campbelli venom has been described. In this work we present a proteomic study of B. myersi ... -
Three-finger toxins from the venom of Micrurus tschudii (desert coral snake): isolation and characterization of tschuditoxin-I
Lomonte, Bruno; Camacho Umaña, Erika; Fernández Ulate, Julián; Salas Arruz, María; Zavaleta Martínez Vargas, Alfonso (2019)Venoms from Micrurus (New World coral snakes) display potent peripheral neurotoxicity which may cause death by respiratory paralysis, yet many are poorly or not characterized. The major venom components of coral snakes are ... -
Understanding structural and functional aspects of PII snake venom metalloproteinases: Characterization of BlatH1, a hemorrhagic dimeric enzyme from the venom of Bothriechis lateralis
Camacho Umaña, Erika; Villalobos Chacón, Eva; Sanz, Libia; Pérez, Alicia; Escalante Muñoz, Teresa; Lomonte, Bruno; Calvete Chornet, Juan José; Gutiérrez, José María; Rucavado Romero, Alexandra (2014-06)A new homodimeric PII metalloproteinase, named BlatH1, was purified from the venom of the Central American arboreal viperid snake Bothriechis lateralis by a combination of anion-exchange chromatography, hydrophobic interaction ... -
Venom characterization of the bark scorpion Centruroides edwardsii (Gervais 1843): composition, biochemical activities and in vivo toxicity for potential prey
Díaz Oreiro, Cecilia; Rivera Hidalgo, Jennifer; Lomonte, Bruno; Bonilla Murillo, Fabián; Diego García, Elia; Camacho Umaña, Erika; Tytgat, Jan; Sasa Marín, Mahmood (2019)In this study, we characterize the venom of Centruroides edwardsii, one of the most abundant scorpions in urban and rural areas of Costa Rica, in terms of its biochemical constituents and their biological activities. C. ... -
Venomics of the Central American Lyre snake Trimorphodon quadruplex (Colubridae: Smith, 1941) from Costa Rica
Mackessy, Stephen P.; Bryan, Wendy; Smith, Cara F.; Lopez, Keira; Fernández Ulate, Julián; Bonilla Murillo, Fabián; Camacho Umaña, Erika; Sasa Marín, Mahmood; Lomonte, Bruno (2020)Rear-fanged colubrid snakes include hundreds of species globally that possess a Duvernoy's venom gland and often one-several enlarged rear maxillary teeth. We investigated the venom proteome of the Central American Lyre ...