Browsing Microbiología by Author "Calvete Chornet, Juan José"
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A bright future for integrative venomics
Calvete Chornet, Juan José; Lomonte, Bruno (2015-10)Venomous secretions are produced by a myriad of animal species, from invertebrates to vertebrates. As a general rule, peptides and proteins represent the most abundant and functionally relevant components of these dangerous ... -
A Call for Incorporating Social Research in the Global Struggle against Snakebite
Gutiérrez, José María; Burnouf, Thierry; Harrison, Robert A.; Calvete Chornet, Juan José; Brown, Nicholas I.; Jensen, Simon D.; Warrell, David A.; Williams, David J. (2015-09-17)In Africa, Asia, Latin America, and parts of Oceania, envenoming after snakebite is a serious public health problem. Conservative data suggest that between 1.2 and 5.5 million people suffer snakebites every year, resulting ... -
A multicomponent strategy to improve the availability of antivenom for treating snakebite envenoming
Gutiérrez, José María; Burnouf, Thierry; Harrison, Robert A.; Calvete Chornet, Juan José; Kuch, Ulrich; Warrell, David A.; Williams, David J. (2014-07-01)Snakebite envenoming is a common but neglected public health problem, particularly in impoverished rural regions of sub-Saharan Africa, Asia and Latin America. The only validated treatment for this condition is passive ... -
A novel pentameric phospholipase A2 myotoxin (PophPLA2) from the venom of the pit viper Porthidium ophryomegas
Vindas González, Julio; Carrera Rengifo, Yarina; Lomonte, Bruno; Gutiérrez, José María; Calvete Chornet, Juan José; Sanz, Libia; Fernández Ulate, Julián (2018-06-07)The first toxin isolated from the venomous pit viper Porthidiumophryomegas is a basic pentameric phospholipase A2 (PophPLA2). Elucidation of its amino acid sequence showed that it belongs to the group IIA of secreted ... -
Amino acid sequence and biological characterization of BlatPLA2, a non-toxic acidic phospholipase A2 from the venom of the arboreal snake Bothriechis lateralis from Costa Rica
Van der Laat Villalobos, Marco; Fernández Ulate, Julián; Durban, Jordi; Villalobos Chacón, Eva; Camacho Umaña, Erika; Calvete Chornet, Juan José; Lomonte, Bruno (2013-10)Bothriechis is considered a monophyletic, basal genus of arboreal Neotropical pitvipers distributed across Middle America. The four species found in Costa Rica (B. lateralis, B. schlegeli, B. nigroviridis, B. supraciliaris) ... -
Antivenomic Assessment of the Immunological Reactivity of EchiTAb-Plus-ICP, an Antivenom for the Treatment of Snakebite Envenoming in Sub-Saharan Africa
Calvete Chornet, Juan José; Cid, Pedro; Sanz, Libia; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Herrera Vega, María; León Montero, Guillermo; Harrison, Robert A.; Durfa, Nandul; Nasidi, Abdulsalami; Theakston, R. David G.; Warrell, David A.; Gutiérrez, José María (2010)The immunoreactivity of EchiTAb-Plus-ICP, an antivenom developed for the treatment of snakebite envenoming in sub-Saharan Africa, to venoms of seven Echis and Bitis species, was assessed by “antivenomics.” This proteomic ... -
Antivenomics of Atropoides mexicanus and Atropoides picadoi snake venoms: Relationship to the neutralization of toxic and enzymatic activities
Antúnez Oliva, José; Fernández Ulate, Julián; Lomonte, Bruno; Angulo Ugalde, Yamileth; Sanz, Libia; Pérez, Alicia; Calvete Chornet, Juan José; Gutiérrez, José María (2010-09-30)Viperid snakes of the genus Atropoides are distributed in Mexico and Central America and, owing to their size and venom yield, are capable of provoking severe envenomings in humans. This study evaluated, using an ‘antivenomics’ ... -
Antivenomics: a proteomics tool for studying the immunoreactivity of antivenoms
Calvete Chornet, Juan José; Gutiérrez, José María; Sanz, Libia; Pla Ferrer, Davinia; Lomonte, Bruno (2015-03)Envenoming following snakebite is largely a neglected threat to public health in tropical and subtropical regions of Africa, Asia, Latin America and Oceania addicting some of the world’s poorest rural communities. An ... -
Assessing the preclinical efficacy of antivenoms: From the lethality neutralization assay to antivenomics
Gutiérrez, José María; Solano Trejos, María Gabriela; Pla Ferrer, Davinia; Herrera Vega, María; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Sanz, Libia; Lomonte, Bruno; Calvete Chornet, Juan José; León Montero, Guillermo (2013-07)The assessment of the capacity of antivenoms to neutralize the lethal activity of snake venoms is the gold standard in the preclinical analysis of antivenom efficacy, and is routinely performed by manufacturers and quality ... -
Characterization of a novel snake venom component: Kazal-type inhibitor-like protein from the arboreal pitviper Bothriechis schlegelii
Fernández Ulate, Julián; Gutiérrez, José María; Calvete Chornet, Juan José; Sanz, Libia; Lomonte, Bruno (2016-06)Snake venoms are composed mainly of a mixture of proteins and peptides. Notably, all snake venom toxins have been assigned to a small number of protein families. Proteomic studies on snake venoms have recently identified ... -
Combined venom gland cDNA sequencing and venomics of the New Guinea small-eyed snake, Micropechis ikaheka
Paiva, Owen K.; Pla Ferrer, Davinia; Wright, Christine E.; Beutler, Markus; Sanz, Libia; Gutiérrez, José María; Williams, David J.; Calvete Chornet, Juan José (2014-10-14)The venom arsenal of the New Guinea small-eyed snake, Micropechis ikaheka, was investigated by a joint cDNA sequencing and venomics approach. Twenty-seven full-length DNA sequences encoding novel venom proteins were recovered ... -
Combined venomics, venom gland transcriptomics, bioactivities, and antivenomics of two Bothrops jararaca populations from geographic isolated regions within the Brazilian Atlantic rainforest
Gonçalves Machado, Larissa; Pla Ferrer, Davinia; Sanz, Libia; Jorge, Roberta Jeane B.; Leitão De Araújo, Moema; Alves, Maria Lúcia M.; Alvares, Diego Janisch; De Miranda, Joari; Nowatzki, Jenifer; de Morais Zani, Karen; Fernandes, Wilson; Tanaka Azevedo, Anita Mitico; Fernández Ulate, Julián; Zingali, Russolina B.; Gutiérrez, José María; Corrêa Netto, Carlos; Calvete Chornet, Juan José (2016-03-01)Bothrops jararaca is a slender and semi-arboreal medically relevant pit viper species endemic to tropical and subtropical forests in southern Brazil, Paraguay, and northern Argentina (Misiones). Within its geographic range, ... -
Comparative biochemical characterization of Viperidae snake venoms from Perú reveals two compositional patterns of phospholipase A2 expression
Lomonte, Bruno; Díaz Oreiro, Cecilia; Chaves Mora, Fernando; Fernández Ulate, Julián; Ruiz, Marco; Salas, María; Zavaleta Martínez Vargas, Alfonso; Calvete Chornet, Juan José; Sasa Marín, Mahmood (2020)Snake species within the Bothrops complex (sensu lato) are of medical relevance in Latin America, but knowledge on their venom characteristics is limited, or even unavailable, for some taxa. Perú harbors 17 species of pit ... -
Comparative proteomic analysis of the venom of the taipan snake, Oxyuranus scutellatus, from Papua New Guinea and Australia: Role of neurotoxic and procoagulant effects in venom toxicity
Herrera Vega, María; Fernández Ulate, Julián; Vargas Arroyo, Mariángela; Villalta Arrieta, Mauren; Segura Ruiz, Álvaro; León Montero, Guillermo; Angulo Ugalde, Yamileth; Paiva, Owen K.; Matainaho, Teatulohi; Jensen, Simon D.; Winkel, Kenneth D.; Calvete Chornet, Juan José; Williams, David J.; Gutiérrez, José María (2012-04-03)The venom proteomes of populations of the highly venomous taipan snake, Oxyuranus scutellatus, from Australia and Papua New Guinea (PNG), were characterized by reverse-phase HPLC fractionation, followed by analysis of ... -
Comparative study of the cytolytic activity of snake venoms from African spitting cobras (Naja spp., Elapidae) and its neutralization by a polyspecific antivenom
Méndez Madriz, Ileana Sofía; Gutiérrez, José María; Angulo Ugalde, Yamileth; Calvete Chornet, Juan José; Lomonte, Bruno (2011-11)Venoms of several Naja species found in Sub-Saharan Africa, and commonly known as "spitting cobras", induce a predominantly cytotoxic pattern of envenomings that may evolve into tissue necrosis and gangrene. Cytotoxic ... -
Danger in the canopy: comparative proteomics and bioactivities of the venoms of the South American palm viper Bothrops bilineatus subspecies bilineatus and smaragdinus, and antivenomics of B. b. bilineatus (Rondônia) venom against the Brazilian pentabothropic antivenom
Sanz, Libia; Quesada Bernat, Sarai; Pérez, Alicia; de Morais Zani, Karen; SantˈAnna, Sávio S.; Hatakeyama, Daniela M.; Tasima, Lidia J.; de Souza, Moisés B.; Kayano, Anderson M.; Zavaleta Martínez Vargas, Alfonso; Salas, María; Soares, Andreimar Martins; Calderón, Leonardo de A.; Tanaka Azevedo, Anita Mitico; Lomonte, Bruno; Calvete Chornet, Juan José; Caldeira, Cleópatra A. S. (2020)We report a structural and functional proteomics characterization of venoms of the two subspecies (Bothrops bilineatusbilineatus and B. b. smaragdinus) of the South American palm pit viper from the Brazilian state of ... -
Ending the drought: New strategies for improving the flow of affordable, effective antivenoms in Asia and Africa
Williams, David J.; Gutiérrez, José María; Calvete Chornet, Juan José; Wüster, Wolfgang; Ratanabanangkoon, Kavi; Paiva, Owen K.; Brown, Nicholas I.; Casewell, Nicholas R.; Harrison, Robert A.; Rowley, Paul D.; O'Shea, Mark; Jensen, Simon D.; Winkel, Kenneth D.; Warrell, David A. (2011-08-24)The development of snake antivenoms more than a century ago should have heralded effective treatment of the scourge of snakebite envenoming in impoverished, mostly rural populations around the world. That snakebite still ... -
Evaluation of the preclinical efficacy of four antivenoms, distributed in sub-Saharan Africa, to neutralize the venom of the carpet viper, Echis ocellatus, from Mali, Cameroon, and Nigeria
Sánchez Céspedes, Laura Vanessa; Pla Ferrer, Davinia; Herrera Vega, María; Chippaux, Jean Philippe; Calvete Chornet, Juan José; Gutiérrez, José María (2015-11)Snakebite envenoming causes a heavy toll in sub-Saharan Africa in terms of mortality and sequelae. In the West African savannah, the viperid Echis ocellatus is responsible for the vast majority of bites. In the last decades, ... -
First crotoxin-like phospholipase A2 complex from a New World non-rattlesnake species: Nigroviriditoxin, from the arboreal Neotropical snake Bothriechis nigroviridis
Lomonte, Bruno; Mora Obando, Diana; Fernández Ulate, Julián; Sanz, Libia; Pla Ferrer, Davinia; Gutiérrez, José María; Calvete Chornet, Juan José (Toxicon vol 93:144–154, 2015-01)Bothriechis nigroviridis is an arboreal Neotropical pitviper found in Costa Rica and Panam a. A previous proteomic profiling of its venom revealed the presence of proteins with homology to the A and B subunits of ... -
Identification of New Snake Venom Metalloproteinase Inhibitors Using Compound Screening and Rational Peptide Design
Villalta Romero, Fabián; Gortat, Anna; Herrera, Andrés E.; Arguedas, Rebeca; Quesada Espinoza, Francisco Javier; Lopes de Melo, Robson; Calvete Chornet, Juan José; Montero Villalobos, Mavis Lili; Murillo Masís, Renato; Rucavado Romero, Alexandra; Pérez Payá, Enrique (2012)The majority of snakebite envenomations in Central America are caused by the viperid species Bothrops asper, whose venom contains a high proportion of zinc-dependent metalloproteinases that play a relevant role in the ...