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Impact of Regional Variation in Bothrops asper Snake Venom on the Design of Antivenoms: Integrating Antivenomics and Neutralization Approaches

Gutiérrez, José María; Sanz, Libia; Flores Díaz, Marietta; Figueroa, Lucía; Madrigal Villalobos, Marvin; Herrera Vega, María; Villalta Arrieta, Mauren; León Montero, Guillermo; Estrada Umaña, Ricardo; Borges, Adolfo; Alape Girón, Alberto; Calvete Chornet, Juan José (2010-10-21)

Intraspecific snake venom variations have implications in the preparation of venom pools for the generation of antivenoms. The impact of such variation in the cross-reactivity of antivenoms against Bothrops asper venom was ...

Pathogenic mechanisms underlying adverse reactions induced by intravenous administration of snake antivenoms

León Montero, Guillermo; Herrera Vega, María; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Gutiérrez, José María (2013-12-15)

Snake antivenoms are formulations of immunoglobulins, or immunoglobulin fragments, purified from the plasma of animals immunized with snake venoms. Their therapeutic success lies in their ability to mitigate the progress ...

Factors associated with adverse reactions induced by caprylic acid-fractionated whole IgG preparations: comparison between horse, sheep and camel IgGs

Herrera Vega, María; León Montero, Guillermo; Segura Ruiz, Álvaro; Meneses, Fabricio; Lomonte, Bruno; Chippaux, Jean Philippe; Gutiérrez, José María (2005-12)

Caprylic acid purification of IgG, currently used in the manufacture of horse-derived antivenoms, was successfully adapted for the preparation of sheep and camel IgG. Sheep IgG had a molecular mass of approximately 150 ...

Comparison of the adjuvant activity of aluminum hydroxide and calcium phosphate on the antibody response towards Bothrops asper snake venom

Olmedo, Hidekel; Herrera Vega, María; Rojas Sánchez, Leonardo; Villalta Arrieta, Mauren; Vargas Arroyo, Mariángela; Leiguez, Elbio; Teixeira, Catarina de Fátima; Estrada Umaña, Ricardo; Gutiérrez, José María; León Montero, Guillermo; Montero Villalobos, Mavis Lili (2014)

The adjuvanticity of aluminum hydroxide and calcium phosphate on the antibody response in mice towards the venom of the snake Bothrops asper was studied. It was found that, in vitro, most of the venom proteins are similarly ...

Toxicity of Bothrops sp snake venoms from Ecuador and preclinical assessment of the neutralizing efficacy of a polyspecific antivenom from Costa Rica

Laines, Johana; Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Herrera Vega, María; Vargas Arroyo, Mariángela; Alvarez, Gladys; Gutiérrez, José María; León Montero, Guillermo (2014-09-15)

The toxicological profile of the venoms of the snakes Bothrops asper and Bothrops atrox from Ecuador was investigated, together with the venom of a population of B. asper formerly classified as ‘Bothrops xanthogrammus’. ...

Pathogenesis of dermonecrosis induced by venom of the spitting cobra, Naja nigricollis: An experimental study in mice

Rivel Piedra, Mario; Solano, Daniela; Herrera Vega, María; Vargas Arroyo, Mariángela; Villalta Arrieta, Mauren; Segura Ruiz, Álvaro (2016-09-01)

The pathogenesis of dermonecrosis induced by the venom of the African spitting cobra Naja nigricollis was investigated in a mouse model. Intradermal injection of venom induced a macroscopic necrotic lesion. Histological ...

Intravenous administration of equine-derived whole IgG antivenom does not induce early adverse reactions in non-envenomed horses and cows

Estrada Umaña, Ricardo; Herrera Vega, María; Segura Ruiz, Álvaro; Araya, Javier; Boschini Figueroa, Carlos; Gutiérrez, José María; León Montero, Guillermo (2010-11)

Administration of antivenoms to treat snakebite envenomings has the potential risk of inducing early adverse reactions. The mechanisms involved in these reactions are unclear. In this study, polyspecific antivenom consisting ...

Study of the design and analytical properties of the lethality neutralization assay used to estimate antivenom potency against Bothrops asper snake venom

Solano Trejos, María Gabriela; Segura Ruiz, Álvaro; Herrera Vega, María; Gómez Argüello, Aarón; Villalta Arrieta, Mauren; Gutiérrez, José María; León Montero, Guillermo (2010-09)

The lethality neutralization assay performed in mice is the standard recommended by the World Health Organization to estimate antivenom potency. The interpretation of its results without considering its analytical capacity ...

Preclinical assessment of the efficacy of a new antivenom (EchiTAb-Plus-ICP) for the treatment of viper envenoming in sub-Saharan Africa

Segura Ruiz, Álvaro; Villalta Arrieta, Mauren; Herrera Vega, María; León Montero, Guillermo; Harrison, Robert A.; Durfa, Nandul; Nasidi, Abdulsalami; Calvete Chornet, Juan José; Theakston, R. David G.; Warrell, David A.; Gutiérrez, José María (2010-02)

A preclinical assessment was performed on the neutralizing efficacy of a whole IgG polyspecific antivenom (EchiTAb-Plus-ICP®), designed for the treatment of snakebite envenomings in Nigeria. It was generated by immunizing ...

Development of a chicken-derived antivenom against the taipan snake (Oxyuranus scutellatus) venom and comparison with an equine antivenom

Navarro Arias, Diego; Vargas Arroyo, Mariángela; Herrera Vega, María; Segura Ruiz, Álvaro; Gómez Argüello, Aarón; Villalta Arrieta, Mauren; Ramírez Arguedas, Nils Antonio; Williams, David J.; Gutiérrez, José María; León Montero, Guillermo (2016-09-15)

A chicken-derived antivenom (ChDAv) towards taipan snake (Oxyuranus scutellatus) venom was produced by purifying anti-taipan IgY from egg yolks of hens immunized with taipan venom. The productivity, antivenomic profile, ...