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dc.creatorLomonte, Bruno
dc.creatorGutiérrez, José María
dc.creatorBorkow, Gadi
dc.creatorOvadia, Michael
dc.creatorTarkowski, Andrej
dc.creatorHanson, Lars Ake
dc.date.accessioned2016-11-11T15:05:40Z
dc.date.available2016-11-11T15:05:40Z
dc.date.issued1994-04
dc.identifier.issn0041-0101
dc.identifier.urihttps://hdl.handle.net/10669/29231
dc.description.abstractIn vivo, hemorrhagic toxins isolated from snake venoms cause a disorganization of the basal lamina of capillaries, with a concomitant degenerative process of endothelial cells. In this study we investigated the effects of BaH-1, a hemorrhagic metalloproteinase purified from the venom of Bothrops asper, on a murine endothelial cell line of capillary origin. A quantitative cytotoxicity assay based on the release of lactic dehydrogenase was utilized. BaH-1, despite its potent hemorrhagic activity, did not exert direct cytolytic activity on the endothelial cells, even at concentrations as high as 65 micrograms/ml. The only visible effect of BaH-1 on the cultured cells was a relatively slow, moderate detachment of cells, interpreted as a consequence of proteolytic degradation of extracellular matrix components. In contrast, myotoxin II, a lysine-49 phospholipase A2 from the same venom, was clearly cytotoxic to this cell type, albeit being devoid of hemorrhagic activity. These findings suggest that the ability of venom metalloproteinases to induce hemorrhage is not related to a direct cytotoxic action on endothelial cells, and that the rapid degenerative changes of endothelium observed in vivo are probably the result of an indirect mechanism.es_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 32, Número 2, 1994es_ES
dc.subjectAnimalses_ES
dc.subjectBothropses_ES
dc.subjectCells, Culturedes_ES
dc.subjectCrotalid Venomses_ES
dc.subjectEndothelium, Vasculares_ES
dc.subjectGroup II Phospholipases A2es_ES
dc.subjectHemorrhagees_ES
dc.subjectL-Lactate Dehydrogenasees_ES
dc.subjectMetalloendopeptidaseses_ES
dc.subjectMicees_ES
dc.subjectNeurotoxinses_ES
dc.subjectPhospholipases Aes_ES
dc.subjectPhospholipases A2es_ES
dc.subjectReptilian Proteinses_ES
dc.subjectSnake venomes_ES
dc.titleActivity of hemorrhagic metalloproteinase BaH-1 and myotoxin II from Bothrops asper snake venom on capillary endothelial cells in vitroes_ES
dc.typeartículo original
dc.identifier.doi10.1016/0041-0101(94)90302-6es_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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