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dc.creatorCalvete Chornet, Juan José
dc.creatorSanz, Libia
dc.creatorCid, Pedro
dc.creatorDe La Torre, Pilar
dc.creatorFlores Díaz, Marietta
dc.creatorDos Santos, María Cristina
dc.creatorBorges, Adolfo
dc.creatorBremo, Adolfo
dc.creatorAngulo Ugalde, Yamileth
dc.creatorLomonte, Bruno
dc.creatorAlape Girón, Alberto
dc.creatorGutiérrez, José María
dc.date.accessioned2016-11-29T15:06:51Z
dc.date.available2016-11-29T15:06:51Z
dc.date.issued2010
dc.identifier.citationhttp://pubs.acs.org/doi/abs/10.1021/pr9008749es_ES
dc.identifier.issn1535-3907
dc.identifier.urihttp://hdl.handle.net/10669/29333
dc.description.abstractWe report a comparative venomic and antivenomic characterization of the venoms of newborn and adult specimens of the Central American rattlesnake, Crotalus simus, and of the subspecies cumanensis, durissus, ruruima, and terrificus of South American Crotalus durissus. Neonate and adult C. simus share about 50% of their venom proteome. The venom proteome of 6-week-old C. simus is predominantly made of the neurotoxic heterodimeric phospholipase A2 (PLA2 crotoxin) (55.9%) and serine proteinases (36%), whereas snake venom Zn2+-metalloproteinases (SVMPs), exclusively of class PIII, represent only 2% of the total venom proteins. In marked contrast, venom from adult C. simus comprises toxins from 7 protein families. A large proportion (71.7%) of these toxins are SVMPs, two-thirds of which belong to the PIII class. These toxin profiles correlate well with the overall biochemical and pharmacological features of venoms from adult (hemorrhagic) and newborn (neurotoxic) C. simus specimens. The venoms of the South American Crotalus subspecies belong to one of two distinct phenotypes. C. d. cumanensis exhibits high levels of SVMPs and low lethal potency (LD50), whereas C. d. subspecies terrificus, ruruima, and durissus have low SVMP activity and high neurotoxicity to mice. Their overall toxin compositions explain the outcome of envenomation by these species. Further, in all C. simus and C. durissus venoms, the concentration of neurotoxins (crotoxin and crotamine) is directly related with lethal activity, whereas lethality and metalloproteinase activity show an inverse relationship. The similar venom toxin profiles of newborn C. simus and adult C. durissus terrificus, ruruima, and durissus subspecies strongly suggests that the South American taxa have retained juvenile venom characteristics in the adult form (paedomorphism) along their North−South stepping-stone dispersal. The driving force behind paedomorphism is often competition or predation pressure. The increased concentration of the neurotoxins crotoxin and crotamine in South American rattlesnake venoms strongly argues that the gain of neurotoxicity and lethal venom activities to mammals may have represented the key axis along which overall venom toxicity has evolved during Crotalus durissus invasion of South America. The paedomorphic trend is supported by a decreasing LNC (lethal neurotoxicity coefficient, defined as the ratio between the average LD50 of the venom and the crotoxin + crotamine concentration) along the North−South axis, coincident with the evolutionary dispersal pattern of the Neotropical rattlesnakes. The indistinguisable immunoreactivity patterns of Costa Rican and Venezuelan polyvalent antivenoms toward C. simus and C. durissus venoms strongly suggest the possibility of using these antivenoms indistinctly for the management of snakebites by adult C. simus and by certain C. d. cumanensis populations exhibiting a hemorrhagic venom phenotype. The antivenomic results also explain why the antivenoms effectively neutralize the hemorrhagic activity of adult C. simus venoms but does not protect against adult C. durissus sp. and newborn C. simus envenomations. The identification of evolutionary trends among tropical Crotalus, as reported here, may have an impact in defining the mixture of venoms for immunization to produce an effective pan-American anti-Crotalus antivenom.es_ES
dc.description.sponsorshipMinisterio de Educación y Ciencia/[BFU2007-6156]//Españaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A7-611]/UCR/Costa Ricaes_ES
dc.description.sponsorshipPrograma Iberoamericano de Ciencia y Tecnología para el Desarrollo/206AC0281/CYTED/Españaes_ES
dc.description.sponsorshipAcciones Integradas/2006CR001//es_ES
dc.description.sponsorshipConsejo Superior de Investigaciones Científicas/[2007CR0004]/CRUSA-CSIC/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceJournal Proteome Research; Volumen 9, Número 1. 2010es_ES
dc.subjectAntibothropic and anticrotalic ABC antivenomes_ES
dc.subjectAntivenomicses_ES
dc.subjectCrotalus durissuses_ES
dc.subjectCrotalus simuses_ES
dc.subjectCrotoxines_ES
dc.subjectOntogenetic shiftes_ES
dc.subjectPaedomorphismes_ES
dc.subjectPolyvalent (Crotalinae) ICP antivenomes_ES
dc.subjectSnake venom protein familieses_ES
dc.subjectSnake venomicses_ES
dc.subjectSnake venomes_ES
dc.titleSnake Venomics of the Central American Rattlesnake Crotalus simus and the South American Crotalus durissus Complex Points to Neurotoxicity as an Adaptive Paedomorphic Trend along Crotalus Dispersal in South Americaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1021/pr9008749
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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