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dc.creatorZuliani, Juliana Pavan
dc.creatorFernandes, Cristina Maria
dc.creatorZamunér, Stella Regina
dc.creatorGutiérrez, José María
dc.creatorTeixeira, Catarina de Fátima
dc.date.accessioned2017-01-18T15:10:19Z
dc.date.available2017-01-18T15:10:19Z
dc.date.issued2005-03
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010104004532es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/29433
dc.description.abstractThe inflammatory events induced in the peritoneal cavity of mice by two PLA2s isolated from Bothrops asper snake venom were investigated. MT-III, an Asp-49 catalytically active enzyme and MT-II, a catalytically inactive Lys-49 variant induced increase in vascular permeability. Inhibition of enzymatic activity of MT-III with p-bromophenacyl bromide reduced this effect. MT-III induced a larger neutrophil infiltrate than MT-II. This activity was significantly reduced after inhibition of catalytic activity. Reduction in the number of neutrophils was observed when antibodies against l-selectin, CD18 or LFA-1 were used, suggesting the involvement of these adhesion molecules in the effects of both PLA2s. There was no effect with antibodies against ICAM-1 and PECAM-1. Increase in the levels of LTB4 and TXA2, as well as of IL-1, IL-6 and TNF-α, were observed in the peritoneal exudates induced by MT-III. MT-II did not enhance levels of eicosanoids but increased those of cytokines. It is concluded that both PLA2s induce inflammatory events in this model. Since MT-III exerts a stronger proinflammatory effect, the enzymatic phospholipid hydrolysis may be relevant for these phenomena. However, the fact that MT-II induced inflammation suggests that molecular regions distinct from the catalytic site elicit inflammatory events perhaps by interacting with specific cell membrane acceptors.es_ES
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo/[98/00162-9]/FAPESP/Brasiles_ES
dc.description.sponsorshipMinistério da Ciência, Tecnologia, Inovações e Comunicações/[301199/91-4]/CNPq/Brasiles_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 45, Número 3, 2005es_ES
dc.subjectBothrops Asperes_ES
dc.subjectInflammationes_ES
dc.subjectLeukocyteses_ES
dc.subjectAdhesion Moleculeses_ES
dc.subjectEicosanoidses_ES
dc.subjectCytokineses_ES
dc.subjectSnake venomes_ES
dc.titleInflammatory events induced by Lys-49 and Asp-49 phospholipases A2 isolated from Bothrops asper snake venom: role of catalytic activityes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2004.11.004
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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