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dc.creatorRucavado Romero, Alexandra
dc.creatorFlores Sánchez, Eladio
dc.creatorFranceschi, Aida
dc.creatorMagalhaes, Arinos
dc.creatorGutiérrez, José María
dc.date.accessioned2017-01-23T16:48:25Z
dc.date.available2017-01-23T16:48:25Z
dc.date.issued1999-09
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010198002682
dc.identifier.issn0041-0101
dc.identifier.urihttps://hdl.handle.net/10669/29451
dc.description.abstractLocal tissue damage induced by LHF-II, a 22-kDa hemorrhagic metalloproteinase from Lachesis muta venom was studied. Intravital microscopy experiments evidenced hemorrhagic events 2 min after LHF-II application onto cremaster muscle, characterized by microhemorrhages in capillary vessels and venules. However, histological analysis showed only mild hemorrhage in the gastrocnemius muscle. LHF-II degraded laminin, fibronectin and type IV collagen upon incubation in vitro, but was not cytotoxic to capillary endothelial cells in culture. Intramuscular injection of LHF-II induced a mild myonecrosis, with early small increments in plasma creatine kinase activity. It also induced edema in the mouse footpad at doses where hemorrhage is absent. Injection of LHF-II induced the synthesis of matrix metalloproteinases evidenced in muscle homogenates and in exudate samples. It is concluded that LHF-II has weak hemorrhagic and myotoxic activities, and that its role in the pathogenesis of L. muta-induced local tissue damage is associated with edema formation and degradation of extracellular matrix components, either directly or by activation of endogenous matrix metalloproteinases.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-98-202]/UCR/Costa Ricaes_ES
dc.description.sponsorshipInternational Foundation for Science/[F/2707-1]/IFS/Sueciaes_ES
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico//CNPq/Brasiles_ES
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais//FAPEMIG/Brasiles_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 37, Número 9, 1999es_ES
dc.subjectAnimalses_ES
dc.subjectCapillarieses_ES
dc.subjectCells, Culturedes_ES
dc.subjectCreatine Kinasees_ES
dc.subjectEdemaes_ES
dc.subjectExtravasation of Diagnostic and Therapeutic Materialses_ES
dc.subjectFootes_ES
dc.subjectHemorrhagees_ES
dc.subjectInjections, Intramusculares_ES
dc.subjectMetalloendopeptidaseses_ES
dc.subjectMicees_ES
dc.subjectMicrocirculationes_ES
dc.subjectMuscle, Skeletales_ES
dc.subjectNecrosises_ES
dc.subjectPeptide Hydrolaseses_ES
dc.subjectPhospholipases Aes_ES
dc.subjectVenuleses_ES
dc.subjectViperidaees_ES
dc.subjectSnake venomes_ES
dc.titleCharacterization of the local tissue damage induced by LHF-II, a metalloproteinase with weak hemorrhagic activity isolated from Lachesis muta muta snake venomes_ES
dc.typeartículo original
dc.identifier.doi10.1016/S0041-0101(98)00268-2
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-98-202


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