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dc.creatorLopes Ferreira, Mónica
dc.creatorNúñez, Javier
dc.creatorRucavado Romero, Alexandra
dc.creatorFarsky, Sandra H. P.
dc.creatorLomonte, Bruno
dc.creatorAngulo Ugalde, Yamileth
dc.creatorMoura Da Silva, Ana M.
dc.creatorGutiérrez, José María
dc.date.accessioned2017-01-31T19:34:40Z
dc.date.available2017-01-31T19:34:40Z
dc.date.issued2001-02
dc.identifier.citationhttp://onlinelibrary.wiley.com/doi/10.1046/j.1365-2613.2001.00181.x/abstractes_ES
dc.identifier.issn1365-2613
dc.identifier.urihttp://hdl.handle.net/10669/29477
dc.description.abstractStings by Thalassophryne nattereri are responsible for envenomation of fishermen in north-eastern Brazil. Its venom induces prominent local tissue damage, characterized by pain, oedema and necrosis. The pathogenesis of acute muscle damage induced by T. nattereri venom was studied in mice. Intramuscular injection induced myonecrosis within the first hours. Some muscle cells presented a hypercontracted morphology, but most necrotic fibres were not hypercontracted, being instead characterized by a disorganization of myofibrils, with Z line loss, mitochondrial swelling and sarcolemmal disruption. In addition, thrombosis was observed histologically in venules and veins, together with vascular congestion and stasis, evidenced by intravital microscopy. Venom induced a rapid increment in serum creatine kinase (CK) levels, concomitant with a reduction in gastrocnemius muscle CK activity, whereas no increments in muscle lactic acid were detected. A rapid cytolytic effect was induced by the venom on C2C12 murine myoblasts in culture. The inflammatory reaction in affected muscle was characterized by oedema and scarce cellular infiltrate of polymorphonuclear leucocytes and macrophages, with a consequent delay in the removal of necrotic material. Skeletal muscle regeneration was partially impaired, as evidenced by the presence of regenerating fibres of variable size and by the increase of fibrotic tissue in endomysium and perimysium. It is suggested that T. nattereri venom affects muscle fibres by a direct cytotoxic effect, and that the vascular alterations described preclude a successful regenerative process.es_ES
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo/[98/11312-1]/FAPESP/Brasiles_ES
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico/[520636/96]/CNPq/Brasiles_ES
dc.description.sponsorshipUniversidad de Costa Rica//UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceInternational Journal of Experimental Pathology; Volumen 82, Número 1, 2001es_ES
dc.subjectThalassophryne nattereries_ES
dc.subjectMyonecrosises_ES
dc.subjectRegenerationes_ES
dc.subjectThrombosises_ES
dc.subjectFish venomes_ES
dc.titleSkeletal muscle necrosis and regeneration after injection of Thalassophryne nattereri (niquim) fish venom in micees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1046/j.1365-2613.2001.00181.x
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid11422541


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