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dc.creatorLeón Montero, Guillermo
dc.creatorLomonte, Bruno
dc.creatorGutiérrez, José María
dc.date.accessioned2017-02-22T20:08:11Z
dc.date.available2017-02-22T20:08:11Z
dc.date.issued2005-01
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S0041010104003381es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/29550
dc.description.abstractEarly adverse reactions occur in a number of patients treated with heterologous antivenoms and have been associated with anticomplementary activity (ACA). In order to reduce the ACA of equine whole IgG antivenoms produced by caprylic acid fractionation, three different fractionation protocols were compared: (a) routine caprylic acid fractionation; (b) caprylic acid fractionation followed by β-propiolactone treatment; and (c) caprylic acid fractionation followed by ion-exchange chromatography using a quaternary ammonium membrane. The three protocols yielded products with similar physicochemical characteristics and anti-Bothrops asper venom antibody titers, except that ion-exchange purified antivenom had a lower protein concentration. Antivenoms fractionated by using β-propiolactone or filtration through quaternary ammonium membrane had a significantly reduced in vitro ACA. A preparation of caprylic acid-fractionated antivenom was heated in order to induce the formation of protein aggregates; however, its ACA was similar to non-heated antivenom. None of the antivenoms affected the hemolytic activity of serum complement in rabbits after a bolus intravenous administration. It is concluded that (a) β-propiolactone and quaternary ammonium membranes significantly reduce in vitro ACA of caprylic acid-fractionated equine antivenom, and (b) the validity of in vitro ACA as a predictor of EAR needs to be reexamined in clinical and experimental studies, since it may not adequately predict in vivo complement activation by antivenoms.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A1-027]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUnited Nations Educational, Scientific and Cultural Organization/[883.701-3]/UNESCO/es_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 45, Número 1, 2005es_ES
dc.subjectAntivenomes_ES
dc.subjectAnticomplementary Activityes_ES
dc.subjectEarly Adverse Reactionses_ES
dc.subjectIgGes_ES
dc.subjectCH50es_ES
dc.titleAnticomplementary activity of equine whole IgG antivenoms: comparison of three fractionation protocolses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2004.07.025
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-A1-027
dc.identifier.pmid15581691


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