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dc.creatorPla Ferrer, Davinia
dc.creatorGutiérrez, José María
dc.creatorCalvete Chornet, Juan José
dc.date.accessioned2017-03-24T21:34:55Z
dc.date.available2017-03-24T21:34:55Z
dc.date.issued2012-09-15
dc.identifier.citationwww.sciencedirect.com/science/article/pii/S0041010112004497es_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/29621
dc.description2082-09-15 Embargo por política editoriales_ES
dc.description.abstractA second generation antivenomics protocol, based on affinity chromatography, was compared with a previously (first generation) immunodepletion protocol using as a proof of principle the pan-African EchiTAb-Plus-ICP® IgG antivenom and the venoms of Echis ocellatus, Bitis arietans, and African spitting cobras. The antivenom showed qualitatively similar immunoreactivity patterns using either antivenomic approach. Quantitative departures were noticed between both methods, which may be ascribed to differences in calculating the relative amounts of the non-recognized venom proteins. The smoother baseline in chromatograms of the affinity column allowed better resolution and more accurate quantification of the antivenomic outcome than the original immunodepletion protocol. Our results indicate that both methods can be used interchangeably to investigate the in vitro immunoreactivity of antivenoms. However, advantages of the second generation antivenomics are the possibility of analyzing F(ab′)2 antivenoms and the reusability of the affinity columns. These features contribute to the generalization, economy and reproducibility of the method.es_ES
dc.description.sponsorshipMinisterio de Economía y Competitividad/[BFU2010-17373]/MINECO/Españaes_ES
dc.description.sponsorshipGeneralitat Valenciana/[PROMETEO/2010/005]//Españaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A9-003]/UCR/Costa Ricaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B0-519]/UCR/Costa Ricaes_ES
dc.description.sponsorshipConsejo Superior de Investigaciones Científicas/2009CR0021/CRUSA-CSIC/Españaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon;Volumen 60, Número 4. 2012es_ES
dc.subjectSnake antivenomes_ES
dc.subjectAntivenomicses_ES
dc.subjectImmunodepletiones_ES
dc.subjectImmunoaffinity protocoles_ES
dc.subjectEchis ocellatuses_ES
dc.subjectBitis arietanses_ES
dc.subjectAfrican spitting cobraes_ES
dc.subjectSnake venomes_ES
dc.titleSecond generation snake antivenomics: Comparing immunoaffinity and immunodepletion protocolses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2012.04.342
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid22561422


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