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An Asp49 Phospholipase A2 from Snake Venom Induces Cyclooxygenase-2 Expression and Prostaglandin E2 Production via Activation of NF-κB, p38MAPK, and PKC in Macrophages
dc.creator | Moreira, Vanessa | |
dc.creator | Lomonte, Bruno | |
dc.creator | Ramirez Vinolo, Marco Aurélio | |
dc.creator | Curi, Rui | |
dc.creator | Gutiérrez, José María | |
dc.creator | Teixeira, Catarina de Fátima | |
dc.date.accessioned | 2017-06-12T21:08:22Z | |
dc.date.available | 2017-06-12T21:08:22Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | https://www.hindawi.com/journals/mi/2014/105879/ | |
dc.identifier.issn | 1466-1861 | |
dc.identifier.other | Article ID 105879 | |
dc.identifier.uri | https://hdl.handle.net/10669/30096 | |
dc.description.abstract | Phospholipases A2 (PLA2) are key enzymes for production of lipid mediators. We previously demonstrated that a snake venom sPLA2 named MT-III leads to prostaglandin (PG)E2 biosynthesis in macrophages by inducing the expression of cyclooxygenase-2 (COX-2). Herein, we explored the molecular mechanisms and signaling pathways leading to these MT-III-induced effects. Results demonstrated that MT-III induced activation of the transcription factor NF-κB in isolated macrophages. By using NF-κB selective inhibitors, the involvement of this factor in MT-III-induced COX-2 expression and PGE2 production was demonstrated. Moreover, MT-III-induced COX-2 protein expression and PGE2 release were attenuated by pretreatment of macrophages with SB202190, and Ly294002, and H-7-dihydro compounds, indicating the involvement of p38MAPK, PI3K, and PKC pathways, respectively. Consistent with this, MT-III triggered early phosphorylation of p38MAPK, PI3K, and PKC. Furthermore, SB202190, H-7-dihydro, but not Ly294002 treatment, abrogated activation of NF-κB induced by MT-III. Altogether, these results show for the first time that the induction of COX-2 protein expression and PGE2 release, which occur via NF-κB activation induced by the sPLA2-MT-III in macrophages, are modulated by p38MAPK and PKC, but not by PI3K signaling proteins. | es_ES |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo/[07/03336-9]/FAPESP/Brasil | es_ES |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo/[02/13863-2]/FAPESP/Brasil | es_ES |
dc.description.sponsorship | Universidad de Costa Rica//UCR/Costa Rica | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Mediators of Inflammation, Volumen 2014 | es_ES |
dc.subject | Animals | es_ES |
dc.subject | Cells, Cultured | es_ES |
dc.subject | Chromones | es_ES |
dc.subject | Cyclooxygenase 2 | es_ES |
dc.subject | Dinoprostone | es_ES |
dc.subject | Imidazoles | es_ES |
dc.subject | Macrophages | es_ES |
dc.subject | Male | es_ES |
dc.subject | Mice | es_ES |
dc.subject | Morpholines | es_ES |
dc.subject | NF-kappa B | es_ES |
dc.subject | Phospholipases A2 | es_ES |
dc.subject | Protein Kinase C | es_ES |
dc.subject | Pyridines | es_ES |
dc.subject | p38 Mitogen-Activated Protein Kinases | es_ES |
dc.subject | Snake venom | es_ES |
dc.title | An Asp49 Phospholipase A2 from Snake Venom Induces Cyclooxygenase-2 Expression and Prostaglandin E2 Production via Activation of NF-κB, p38MAPK, and PKC in Macrophages | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1155/2014/105879 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
dc.identifier.pmid | 24808633 | |
dc.identifier.pmid | PMC3997854 |
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Microbiología [1171]