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dc.creatorCasais e Silva, Luciana Lyra
dc.creatorTeixeira, Catarina de Fátima
dc.creatorLebrun, Ivo
dc.creatorLomonte, Bruno
dc.creatorAlape Girón, Alberto
dc.creatorGutiérrez, José María
dc.date.accessioned2017-06-26T21:35:15Z
dc.date.available2017-06-26T21:35:15Z
dc.date.issued2016-08-26
dc.identifier.citationhttp://www.sciencedirect.com/science/journal/03784274es_ES
dc.identifier.issn0378-4274
dc.identifier.urihttp://hdl.handle.net/10669/30161
dc.description.abstractThe venom of Micrurus lemniscatus, a coral snake of wide geographical distribution in South America, was fractionated by reverse-phase HPLC and the fractions screened for phospholipase A2 (PLA2) activity. The major component of the venom, a PLA2, here referred to as ‘Lemnitoxin’, was isolated and characterized biochemically and toxicologically. It induces myotoxicity upon intramuscular or intravenous injection into mice. The amino acid residues Arg15, Ala100, Asn108, and a hydrophobic residue at position 109, which are characteristic of myotoxic class I phospholipases A2, are present in Lemnitoxin. This PLA2 is antigenically related to M. nigrocinctus nigroxin, Notechis scutatus notexin, Pseudechis australis mulgotoxin, and Pseudonaja textilis textilotoxin, as demonstrated with monoclonal and polyclonal antibodies. Lemnitoxin is highly selective in its targeting of cells, being cytotoxic for differentiated myotubes in vitro and muscle fibers in vivo, but not for undifferentiated myoblasts or endothelial cells. Lemnitoxin is not lethal after intravenous injection at doses up to 2 μg/g in mice, evidencing its lack of significant neurotoxicity. Lemnitoxin displays anticoagulant effect on human plasma and proinflammatory activity also, as it induces paw edema and mast cell degranulation. Thus, the results of this work demonstrate that Lemnitoxin is a potent myotoxic and proinflammatory class I PLA2.es_ES
dc.description.sponsorshipInternational Centre for Genetic Engineering and Biotechnology/[CRP/COS13-01]/ICGEB/Italiaes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B4-100]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicology Letters; Volumen 257. 2016es_ES
dc.subjectMicrurus lemniscatuses_ES
dc.subjectGroup I phospholipase A2es_ES
dc.subjectAmino acid sequencees_ES
dc.subjectMyotoxicityes_ES
dc.subjectCytotoxicityes_ES
dc.subjectInflammationes_ES
dc.titleLemnitoxin, the major component of Micrurus lemniscatus coral snake venom, is a myotoxic and pro-inflammatory phospholipase A2es_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxlet.2016.06.005
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid27282409


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