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Molecular detection of Clostridium difficile on inert surfaces from a Costa Rican hospital during and after an outbreak
dc.creator | Morales, Luisa | |
dc.creator | Rodríguez Sánchez, César | |
dc.creator | Gamboa Coronado, María del Mar | |
dc.date.accessioned | 2017-07-17T19:40:47Z | |
dc.date.available | 2017-07-17T19:40:47Z | |
dc.date.issued | 2016-12-01 | |
dc.identifier.citation | http://www.sciencedirect.com/science/article/pii/S0196655316308252 | |
dc.identifier.issn | 0196-6553 | |
dc.identifier.uri | https://hdl.handle.net/10669/30369 | |
dc.description.abstract | Background: Hospital transmission of Clostridium difficile is fostered by contamination of surfaces and medical equipment with spores highly resistant to disinfectants and regular cleaning procedures. Despite the outbreaks and fatalities that C difficile causes, its epidemiology has not been studied in hospitals from middle- and low-income countries. To tackle this knowledge gap, the detection frequency of C difficile DNA on inert surfaces of a major Costa Rican hospital during and after an outbreak was compared. Methods: We used a presence-absence real-time polymerase chain reaction to detect a fragment of the tpi gene of C difficile on 21 surface samples collected during an outbreak and 54 surface samples taken 2 years later at the same hospital. Results: C difficile DNA was detected in 40% of the 75 environmental samples analyzed. Whereas 71% of the samples collected during the outbreak were positive, only 28% of the samples obtained 2 years after the outbreak gave the same result. This 2.5× ratio was maintained when the comparison was restricted to the wards that were sampled both during and after the outbreak (72% vs 35%, P = .016). Conclusions: Our results show that environmental surfaces in the hospital analyzed are continuously being contaminated with C difficile DNA and that their level of contamination is higher during an outbreak than after it | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | American Journal of Infection Control; Volumen 44, Número 12. 2016 | es_ES |
dc.subject | Clostridium difficile | es_ES |
dc.subject | Outbreak | es_ES |
dc.subject | RT-PCR | es_ES |
dc.subject | Hospital | es_ES |
dc.subject | Surfaces | es_ES |
dc.title | Molecular detection of Clostridium difficile on inert surfaces from a Costa Rican hospital during and after an outbreak | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1016/j.ajic.2016.09.003 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET) | es_ES |
dc.identifier.pmid | 28340959 |
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Microbiología [1171]