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dc.creatorVan Arnam, Ethan B.
dc.creatorRuzzini, Antonio C.
dc.creatorSit, Clarissa S.
dc.creatorHorn, Heidi A.
dc.creatorPinto Tomás, Adrián A.
dc.creatorCurrie, Cameron Robert
dc.creatorClardy, Jon
dc.date.accessioned2017-07-21T14:37:30Z
dc.date.available2017-07-21T14:37:30Z
dc.date.issued2016-11-15
dc.identifier.citationhttp://www.pnas.org/content/113/46/12940#aff-1es_ES
dc.identifier.issn1091-6490
dc.identifier.issn0027-8424
dc.identifier.urihttp://hdl.handle.net/10669/30392
dc.description.abstractThe bacteria harbored by fungus-growing ants produce a variety of small molecules that help maintain a complex multilateral symbiosis. In a survey of antifungal compounds from these bacteria, we discovered selvamicin, an unusual antifungal polyene macrolide, in bacterial isolates from two neighboring ant nests. Selvamicin resembles the clinically important antifungals nystatin A1 and amphotericin B, but it has several distinctive structural features: a noncationic 6-deoxymannose sugar at the canonical glycosylation site and a second sugar, an unusual 4-O-methyldigitoxose, at the opposite end of selvamicin’s shortened polyene macrolide. It also lacks some of the pharmacokinetic liabilities of the clinical agents and appears to have a different target. Whole genome sequencing revealed the putative type I polyketide gene cluster responsible for selvamicin’s biosynthesis including a subcluster of genes consistent with selvamicin’s 4-O-methyldigitoxose sugar. Although the selvamicin biosynthetic cluster is virtually identical in both bacterial producers, in one it is on the chromosome, in the other it is on a plasmid. These alternative genomic contexts illustrate the biosynthetic gene cluster mobility that underlies the diversity and distribution of chemical defenses by the specialized bacteria in this multilateral symbiosis.es_ES
dc.description.sponsorshipNational Institutes of Health/[R01 GM086258]/NIH/Estados Unidoses_ES
dc.description.sponsorshipNational Institutes of Health/[U19 AI09673]/NIH/Estados Unidoses_ES
dc.description.sponsorshipUniversidad de Costa Rica//UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.sourceProceedings of the National Academy of Sciences, Vol. 113, Núm. 46, 2016.es_ES
dc.subjectAntifungales_ES
dc.subjectHorizontal gene transferes_ES
dc.subjectBiosynthesises_ES
dc.subjectSymbiosises_ES
dc.subjectNatural productses_ES
dc.titleSelvamicin, an atypical antifungal polyene from two alternative genomic contextses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1073/pnas.1613285113
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES


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