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dc.creatorTomaszkiewicz, Marta
dc.creatorRangavittal, Samarth
dc.creatorCechova, Monika
dc.creatorCampos Sánchez, Rebeca
dc.creatorFescemyer, Howard W.
dc.creatorHarris, Robert
dc.creatorYe, Danling
dc.creatorO'Brien, Patricia C.M.
dc.creatorChikhi, Rayan
dc.creatorRyder, Oliver A.
dc.creatorFerguson Smith, Malcolm A.
dc.creatorMedvedev, Paul
dc.creatorMakova, Kateryna D.
dc.creatorCampos Sánchez, Rebeca
dc.description.abstractThe mammalian Y Chromosome sequence, critical for studying male fertility and dispersal, is enriched in repeats and palindromes, and thus, is the most difficult component of the genome to assemble. Previously, expensive and labor-intensive BAC-based techniques were used to sequence the Y for a handful of mammalian species. Here, we present a much faster and more affordable strategy for sequencing and assembling mammalian Y Chromosomes of sufficient quality for most comparative genomics analyses and for conservation genetics applications. The strategy combines flow sorting, short- and long-read genome and transcriptome sequencing, and droplet digital PCR with novel and existing computational methods. It can be used to reconstruct sex chromosomes in a heterogametic sex of any species. We applied our strategy to produce a draft of the gorilla Y sequence. The resulting assembly allowed us to refine gene content, evaluate copy number of ampliconic gene families, locate species-specific palindromes, examine the repetitive element content, and produce sequence alignments with human and chimpanzee Y Chromosomes. Our results inform the evolution of the hominine (human, chimpanzee, and gorilla) Y Chromosomes. Surprisingly, we found the gorilla Y Chromosome to be similar to the human Y Chromosome, but not to the chimpanzee Y Chromosome. Moreover, we have utilized the assembled gorilla Y Chromosome sequence to design genetic markers for studying the male-specific dispersal of this endangered species.es_ES
dc.description.sponsorshipNational Science Foundation/[DBI-ABI 0965596]/NSF/Estados Unidoses_ES
dc.description.sponsorshipNational Science Foundation/[DBI-1356529]/NSF/Estados Unidoses_ES
dc.description.sponsorshipNational Science Foundation/[IIS-1453527]/NSF/Estados Unidoses_ES
dc.description.sponsorshipNational Science Foundation/[IIS-1421908]/NSF/Estados Unidoses_ES
dc.description.sponsorshipNational Science Foundation/[CCF-1439057]/NSF/Estados Unidoses_ES
dc.description.sponsorshipNational Institutes of Health/[1T32GM102057-0A1]/NIH/Estados Unidoses_ES
dc.rightsAtribución-NoComercial 3.0 Costa Rica*
dc.sourceGenome Research, Volumen 26, Número 4. 2016es_ES
dc.titleA time- and cost-effective strategy to sequence mammalian Y Chromosomes: an application to the de novo assembly of gorilla Yes_ES
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES

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Atribución-NoComercial 3.0 Costa Rica
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