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dc.creatorLomonte, Bruno
dc.creatorMoreno Frias, Ernesto
dc.creatorTarkowski, Andrej
dc.creatorHanson, Lars Ake
dc.creatorMaccarana, Marco
dc.date.accessioned2017-09-12T20:08:30Z
dc.date.available2017-09-12T20:08:30Z
dc.date.issued1994
dc.identifier.citationhttp://www.jbc.org/content/269/47/29867.abstractes_ES
dc.identifier.issn1083-351X
dc.identifier.urihttp://hdl.handle.net/10669/73141
dc.description.abstractHeparin binds to phospholipase A2 myotoxins from Bothrops asper snake venom, inhibiting their toxic activities. This interaction was investigated using purified myotoxin II, a Lys-49 phospholipase A2 of this venom, and a series of heparin variants, fragments, and other glycosaminoglycans. The binding was correlated to toxin neutralization, using endothelial cells as a target. Myotoxin II binds radiolabeled heparin in solution unselectively, and forms macromolecular complexes with an optimum at a heparin:toxin molar ratio of 1:5. Both O-sulfates and N-sulfates play a role in heparin binding, in the order of importance 2-O-sulfates > 6-Osulfates > N-sulfates. The shortest heparin oligosaccharides interacting with myotoxin II are hexasaccharides. The binding of a neutralizing monoclonal antibody (MAb-3) to myotoxin II was not inhibited by heparin, indicating that the two molecules interact with different sites on the toxin. A synthetic peptide (residues 115-129 in the numbering system of Renetseder et al. (Renetseder, R., Brunie, S., Dijkstra, B. W., Drenth, J., and Sigler, P. B. (1985) J. Biol. Chem. 260, 11627-11634) of myotoxin II displays both heparin-binding and cytolytic activities. It is concluded that heparin neutralizes myotoxin II by binding to a strongly cationic site in the region of residues 115-129, a possible contribution of lysines 36 and 38 suggested by molecular modeling studies. As this cationic region appears to be responsible for the cytolytic activity of the toxin, the present report constitutes the first identification of a cytotoxic region on a phospholipase A2 myotoxin.es_ES
dc.description.sponsorshipSwedish Agency for Research Cooperation with Developing Countries//SAREC/Sueciaes_ES
dc.description.sponsorshipConsejo Nacional para Investigaciones Científicas y Tecnológicas de Costa Rica//CONICIT/Costa Ricaes_ES
dc.description.sponsorshipInternational Foundation for Science/[F/1388-2]/IFS/Sueciaes_ES
dc.description.sponsorshipSwedish Medical Research Council/[2309]//Sueciaes_ES
dc.description.sponsorshipPolysackaridforskning///Sueciaes_ES
dc.language.isoen_USes_ES
dc.sourceJournal of Biological Chemistry 269, 29867-29873 (1994)es_ES
dc.subjectmyotoxines_ES
dc.subjectheparines_ES
dc.subjectSnake venomes_ES
dc.titleNeutralizing interaction between heparins and myotoxin II, a Lys-49 phospholipase A2 from Bothrops asper snake venom. Identification of a heparin-binding and cytolytic toxin region by the use of synthetic peptides and molecular modelinges_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid7961981


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