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dc.creatorLomonte, Bruno
dc.creatorLeón Montero, Guillermo
dc.creatorHanson, Lars Ake
dc.date.accessioned2017-09-29T20:07:13Z
dc.date.available2017-09-29T20:07:13Z
dc.date.issued1996
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/0041010196000797?via%3Dihubes_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/73320
dc.description.abstractThe ability of two antivenoms to Vipera spp., consisting of Fab (Therapeutic Antibodies), or of F(ab′)2 (Zagreb Institute of Immunology) antibody fragments, to neutralize the hemorrhagic activity of Vipera berus snake venom in mice, was compared. First, the neutralizing potency was determined by in vitro preincubation of venom and antivenom, followed by intradermal injection into mice and subsequent measurement of the hemorrhagic area. Both antivenoms had the same anti-hemorrhagic potency, in terms of volume of antivenom/mass of venom, with an estimated ed50 (50% reduction of the effect) of 80 μl antivenom/mg venom. Therefore, no adjustments in dose were necessary for subsequent in vivo comparative experiments. When the antivenoms were administered intravenously into mice immediately after intradermal venom injection, there was no difference in their effectiveness in reducing hemorrhagic lesions, at all doses tested. These results indicate that neutralization of local hemorrhagic activity of V. berus venom in vivo is not improved by the use of the smaller Fab fragments, in comparison to F(ab′)2, when equivalent neutralizing units are injected. However, this does not exclude the possibility that other clinically relevant venom effects may be neutralized with different efficiency by Fab or F(ab′)2 fragments of antibodies.es_ES
dc.description.sponsorshipInternational Foundation for Science/[F/1388-3F]/IFS/Sueciaes_ES
dc.description.sponsorshipSwedish Agency for Research Cooperation with Developing Countries/[]/SAREC/Sueciaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon, Vol. 34, Núm. 10, 1996es_ES
dc.subjectAntivenomes_ES
dc.subjectSnake venomes_ES
dc.subjectVipera beruses_ES
dc.subjectMicees_ES
dc.titleSimilar effectiveness of Fab and F(ab')2 antivenoms in the neutralization of hemorrhagic activity of Vipera berus snake venom in micees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/0041-0101(96)00079-7
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.pmid8931261


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