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dc.creatorJiménez Charris, Eliécer
dc.creatorMontealegre Sánchez, Leonel
dc.creatorSolano Redondo, Luis
dc.creatorCastro Herrera, Fernando
dc.creatorFierro Pérez, Leonardo
dc.creatorLomonte, Bruno
dc.date.accessioned2018-05-03T21:41:53Z
dc.date.available2018-05-03T21:41:53Z
dc.date.issued2016-09-01
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S0041010116301982?via%3Dihubes_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/74608
dc.description.abstractThe Lansberg’s hognose pitviper, Porthidium lansbergii lansbergii, inhabits northern Colombia. A recent proteomic characterization of its venom (J. Proteomics [2015] 114, 287e299) revealed the presence of phospholipases A2 (PLA2) accounting for 16.2% of its proteins. The two most abundant PLA2s were biochemically and functionally characterized. Pllans-I is a basic, dimeric enzyme with a monomer mass of 14,136 Da, while Pllans-II is an acidic, monomeric enzyme of 13,901 Da. Both have Asp49 in their partial amino acid sequences and, accordingly, are catalytically active upon natural or synthetic substrates. Nevertheless, these two enzymes differ markedly in their bioactivities. Pllans-I induces myonecrosis, edema, and is lethal by intracerebroeventricular injection in mice, as well as cytolytic and anticoagulant in vitro. In contrast, Pllans-II is devoid of these effects, except for the induction of a moderate edema. In spite of lacking myotoxicity, Pllans-II enhances the muscle damaging action of Pllans-I in vivo. Altogether, results further illustrate the divergent functional profiles of basic and acidic PLA2s in viperid venoms, and suggest that Pllans-I plays a myotoxic role in envenomings by P. l. lansbergii, whereas Pllans-II, apparently devoid of toxicity, enhances muscle damage caused by Pllans-I.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-B3-760]/UCR/Costa Ricaes_ES
dc.description.sponsorshipPrograma Nacional de Formación de Investigadores/[567-2012]/Colciencias/Colombiaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon, vol. 119, 289-298.es_ES
dc.subjectProteomees_ES
dc.subjectPorthidium lansbergii lansbergiies_ES
dc.subjectMyotoxines_ES
dc.subjectCytotoxines_ES
dc.subjectSnake venomes_ES
dc.subjectPhospholipase A2es_ES
dc.titleDivergent functional profiles of acidic and basic phospholipases A2 in the venom of the snake Porthidium lansbergii lansbergiies_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/j.toxicon.2016.07.006
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-B3-760
dc.identifier.pmid27381371


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