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dc.creatorHun Opfer, Ruchilia Laya
dc.creatorTroyo Rodríguez, Adriana
dc.creatorTaylor Castillo, Mayra Lizeth
dc.creatorBarbieri, Amália R. M.
dc.creatorLabruna, Marcelo B.
dc.date.accessioned2018-06-22T19:55:31Z
dc.date.available2018-06-22T19:55:31Z
dc.date.issued2011-10-05
dc.identifier.citationhttps://www.liebertpub.com/doi/abs/10.1089/vbz.2011.0641es_ES
dc.identifier.issn1530-3667
dc.identifier.issn1557-7759
dc.identifier.urihttp://hdl.handle.net/10669/74979
dc.description.abstractDuring 2010, 15 adult ticks, identified as Amblyomma cajennense, were collected from horses in Cahuita and Turrialba districts, whereas 7 fleas, identified as Ctenocephalides felis, were collected from a dog in San Jose city, Costa Rica. In the laboratory, three A. cajennense specimens, two from Cahuita and one from Turrialba, were individually processed for rickettsial isolation in cell culture, as was a pool of seven fleas. Rickettsiae were successfully isolated and established in Vero cell culture from the three ticks and from a pool of seven fleas in C6/36 cell culture. The three tick isolates were genotypically identified as Rickettsia amblyommii, and the flea isolate was identified as Rickettsia felis through DNA sequencing of portions of the rickettsial genes gltA, ompA, and ompB of each isolate. In addition, other seven ticks were shown to contain rickettsial DNA. Polymerase chain reaction products of at least two of these ticks were sequenced and also showed to correspond to R. amblyommii. Overall, 66.7% (10/15) of the A. cajennense adult ticks were found to be infected with rickettsiae. This is the first report of a successful isolation in cell culture of R. amblyommii and R. felis from Central America.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[803-A8-127]/UCR/Costa Ricaes_ES
dc.description.sponsorshipRed para la Investigación y el Entrenamiento en Enfermedades Tropicales /[9-N-2008]/NeTropica/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.relation.ispartofVector-Borne and Zoonotic Diseases Volumen 11 Número 10
dc.sourceVector-Borne and Zoonotic Diseases, vol.11(10), pp.1395-1397es_ES
dc.subjectRickettsia felises_ES
dc.subjectRickettsia amblyommiies_ES
dc.subjectIsolationes_ES
dc.subjectCtenocephalides felises_ES
dc.subjectCosta Ricaes_ES
dc.subjectAmblyomma cajennensees_ES
dc.subject636.089 medicina veterinariaes_ES
dc.titleFirst Report of the Isolation and Molecular Characterization of Rickettsia amblyommii and Rickettsia felis in Central Americaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.date.updated2018-05-15T19:43:17Z
dc.identifier.doi10.1089/vbz.2011.0641
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Centro de Investigación en Enfermedades Tropicales (CIET)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES
dc.identifier.codproyecto803-A8-127


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