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Improved high-efficiency protocol for somatic embryogenesis in Peach Palm (Bactris gasipaes Kunth) using RITA® temporary immersion system
dc.creator | Heringer, Angelo Schuabb | |
dc.creator | Steinmacher, Douglas A. | |
dc.creator | Fraga, Hugo Pacheco de Freitas | |
dc.creator | Vieira, Leila do Nascimento | |
dc.creator | Montagna, Tiago | |
dc.creator | Quinga, Liliana Alexandra Pila | |
dc.creator | Quoirin, Marguerite Germaine Ghislaine | |
dc.creator | Jiménez García, Víctor | |
dc.creator | Guerra, Miguel Pedro | |
dc.date.accessioned | 2018-08-31T16:51:09Z | |
dc.date.available | 2018-08-31T16:51:09Z | |
dc.date.issued | 2014-11 | |
dc.identifier.citation | https://www.sciencedirect.com/science/article/pii/S0304423814005287 | |
dc.identifier.issn | 0304-4238 | |
dc.identifier.uri | https://hdl.handle.net/10669/75562 | |
dc.description.abstract | Bactris gasipaes Kunth (Arecaceae) is an Amazonian palm cultivated mainly for the production of fruits and heart of palm. Biotechnological tools based on somatic embryogenesis (SE) can be used for improvement and germplasm conservation of this species. The efficiency of different culture systems and culture medium composition were evaluated to optimize the efficiency of SE. The study also evaluated global DNA methylation levels, total protein and starch contents, and alcohol dehydrogenase (ADH) activity. The RITA® bioreactor showed enhanced multiplication of somatic embryos with increased protein, starch levels and ADH activity. Furthermore, a low DNA global methylation rate (27.52%) was observed, suggesting its relationship with the expression of proteins associated with the maturation of somatic embryos and their subsequent conversion to plantlets. Somatic embryo maturation was successfully achieved on Petri dishes and abscisic acid (ABA) supplementation in the culture medium. Chlorophyll pigmented somatic embryos were obtained in Petri dishes containing PGR-free culture medium, and RITA® provided the greatest number of plantlets to be acclimatized after reaching 6-7 cm in length. In the acclimatization step, the best results were obtained with plantlets derived from RITA® after reaching 6-7 cm in length. These new findings shed light on the importance of defining adequate culture systems for different steps of in vitro regenerative protocols and represent a breakthrough in the in vitro morphogenesis process of peach palm, resulting in a large number of somatic plantlets with enhanced physiological and morphological features. | es_ES |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico/CNPq/[478393/2013-0]/CNPq/Brasil | es_ES |
dc.description.sponsorship | Fundação de Amparo a Pesquisa e Inovação do Estado de Santa Catarina/[3770/2012]/FAPESC/Brasil | es_ES |
dc.description.sponsorship | Fundação de Amparo a Pesquisa e Inovação do Estado de Santa Catarina/[2780/2012-4]/FAPESC/Brasil | es_ES |
dc.description.sponsorship | Consejo Nacional de Investigaciones Científicas y Técnicas/[490552/2010-2]/CONICIT/ | es_ES |
dc.language.iso | en_US | es_ES |
dc.relation.ispartof | ||
dc.source | Scientia Horticulturae, Vol.179, pp.284-292 | es_ES |
dc.subject | Somatic embryogenesis | es_ES |
dc.subject | Temporary immersion system | es_ES |
dc.subject | Pejibaye | es_ES |
dc.subject | Abscisic acid | es_ES |
dc.subject | DNA methylation | es_ES |
dc.subject | 631.523 3 Genética agrícola | es_ES |
dc.title | Improved high-efficiency protocol for somatic embryogenesis in Peach Palm (Bactris gasipaes Kunth) using RITA® temporary immersion system | es_ES |
dc.type | artículo original | |
dc.date.updated | 2018-08-09T04:06:27Z | |
dc.identifier.doi | 10.1016/j.scienta.2014.09.041 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Agroalimentarias::Centro para Investigaciones en Granos y Semillas (CIGRAS) | es_ES |
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