Genipa americana and Ageratina anisochroma, two new hosts of Candidatus Phytoplasma asteris in Costa Rica

Villalobos Muller, William; Montero Astúa, Mauricio; Coto Morales, Teresita; Sandoval Carvajal, Izayana; Moreira Carmona, Lisela

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Date

2018-08-23

Author

Villalobos Muller, William

Montero Astúa, Mauricio

Coto Morales, Teresita

Sandoval Carvajal, Izayana

Moreira Carmona, Lisela

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Abstract

We report two new plant species hosts for Candidatus Phytoplasma asteris: Genipa americana (Rubiaceae) and Ageratina anisochroma (Asteraceae). Phytoplasma infections were detected by real-time loop-mediated isothermal amplification and nestedPCR.Consensussequencesfrombothhostsshare99%identitytothe16SrI-BsubgroupusingBLAST;however,potential new subgroups are suggested due to unique RFLP patterns of the 16S rDNA F2nR2 fragment.