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Aplicación del método de coagulación de plasma al estudio ultraestructural de especímenes biológicos
dc.creator | Hernández Chavarría, Francisco | |
dc.creator | Coto, Erika | |
dc.creator | Colmenares, Alirio | |
dc.creator | Moreira Carmona, Lisela | |
dc.date.accessioned | 2018-12-06T17:34:17Z | |
dc.date.available | 2018-12-06T17:34:17Z | |
dc.date.issued | 1991 | |
dc.identifier.citation | https://revistas.ucr.ac.cr/index.php/rbt/article/view/24853 | |
dc.identifier.issn | 2215-2075 | |
dc.identifier.uri | https://hdl.handle.net/10669/76248 | |
dc.description | Copyright © 1991, Revista Biología Tropical | es_ES |
dc.description.abstract | Biological particulate specimens, including Saccharomyces cerevisiae yeast, bovine spermatozoa and human blood cells (normal erythrocytes and leukemic cells) were processed for scanning and transmission electron microscopy using the coagulated plasma technique. The specimens were suspended in frozen and thawed plasma; later, coagulation was induced by adding CaCI2. The clot was cut into small pieces and processed as tissue fragments. The technique is a useful tool when processing biological particulate specimens for electron microscopy. | es_ES |
dc.description.sponsorship | Universidad de Costa Rica/[803-88-418]/UCR/Costa Rica | es_ES |
dc.language.iso | es | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.source | Revista de Biología Tropical, vol. 39(1), pp. 177-180 | es_ES |
dc.subject | Electron microscopy technique | es_ES |
dc.subject | Ultrastructure of biological particulate specimens | es_ES |
dc.subject | 573.156 Plasma | es_ES |
dc.title | Aplicación del método de coagulación de plasma al estudio ultraestructural de especímenes biológicos | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.15517/RBT.V39I1.24853 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM) | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiología | es_ES |
dc.identifier.codproyecto | 803-88-418 |