In vivo neutralization of the dendrotoxin-mediated neurotoxicity of black mamba venom by oligoclonal human IgG monoclonal antibodies
artículo original
Fecha
2018Autor
Laustsen, Andreas Hougaard
Karatt Vellatt, Aneesh
Masters, Edward W.
Arias Oviedo, Ana Silvia
Pus, Urska
Knudsen, Cecilie
Oscoz, Saioa
Slavny, Peter
Griffiths, Daniel T.
Luther, Alice M.
Leah, Rachael A.
Lindholm, Majken
Lomonte, Bruno
Gutiérrez, José María
McCafferty, John
Metadatos
Mostrar el registro completo del ítemResumen
The black mamba (Dendroaspis polylepis) is one of the most feared snake species of the
African savanna. It has a potent, fast-acting neurotoxic venom comprised of dendrotoxins and
α-neurotoxins associated with high fatality in untreated victims. Current antivenoms are both
scarce on the African continent and present a number of drawbacks as they are derived from
the plasma of hyper-immunized large mammals. Here, we describe the development of an
experimental recombinant antivenom by a combined toxicovenomics and phage display
approach. The recombinant antivenom is based on a cocktail of fully human immunoglobulin
G (IgG) monoclonal antibodies capable of neutralizing dendrotoxin-mediated neurotoxicity of
black mamba whole venom in a rodent model. Our results show the potential use of fully
human monoclonal IgGs against animal toxins and the first use of oligoclonal human IgG
mixtures against experimental snakebite envenoming.
External link to the item
10.1038/s41467-018-06086-4Colecciones
- Microbiología [1170]