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dc.creatorMontero Astúa, Mauricio
dc.creatorSaborío Rodríguez, Guido Alonso
dc.creatorChacón Díaz, Carlos
dc.creatorVillalobos Muller, William
dc.creatorRodríguez Solís, Carlos Mario
dc.creatorMoreira Carmona, Lisela
dc.creatorRivera Herrero, Carmen
dc.date.accessioned2019-03-06T20:02:57Z
dc.date.available2019-03-06T20:02:57Z
dc.date.issued2008-08
dc.identifier.citationapsjournals.apsnet.org/doi/abs/10.1094/PDIS-92-8-1249Aes_ES
dc.identifier.issn0191-2917
dc.identifier.urihttp://hdl.handle.net/10669/76678
dc.descriptioncopyright 2008 American Phytopathology Society Journalses_ES
dc.description.abstractOleander (Nerium oleander L.) shrubs presenting mottling, leaf tip and margin scorch, short internodes, defoliation, and branch dieback were observed at different localities in the Central Valley in Costa Rica. Severity of the symptoms ranged widely, and most plants showed both diseased and healthy branches. In severe cases, entire sections of the plant were defoliated. Symptoms resembled those described for oleander leaf scorch (OLS) caused by the bacterium Xylella fastidiosa in the United States (3). This bacterium has been reported in coffee and citrus plants in Costa Rica. Sixty plants from five different places were sampled and tested using ELISA (Agdia Inc., Elkhart, IN) against X. fastidiosa. Thirty-five plants showed absorbance mean value of duplicate wells greater than the mean of control wells plus three times the standard deviation, and therefore were considered positive. Thirty three of the sixty samples were processed for an immunofluorescence assay modified from Carbajal et al. (1) with antibody to X. fastidiosa (Agdia Inc.). Thirteen samples showed fluorescent rod-shaped bacilli with morphology similar to those observed from a pure culture of X. fastidiosa obtained from coffee. Ten of these thirteen samples were positive by ELISA. DNA extracts (2) from three of the oleander plants with high ELISA absorbance values were tested by nested PCR with primer pair 272-1/272-2 followed by the pair 272-1 int/272-2 int (4). Two of the samples were positive for the bacterium and one of the PCR products was cloned and sequenced in both directions (GenBank Accession No. EU009615). The negative (PCR mix) and positive (pure culture of X. fastidiosa isolated from grapevine) controls for nested- PCR were indeed negative and positive, respectively. The BLAST program was used to compare the sequence to the nucleotide collection (nr/nt) and Microbe Assembled Genomes databases in GenBank. All matches corresponded to X. fastidiosa sequences. The sequence showed 97% similarity with strains Found-4 (coffee strain from Brazil) and Found-5 (citrus strain from Brazil) and 96% similarity with strain Ann-1 from oleander in California. On the basis of serological, microscopic, and molecular detection of X. fastidiosa from oleander exhibiting symptoms of OLS similar to those reported in the literature, this pathogen likely is causing the symptoms we observed in Costa Ricaes_ES
dc.description.sponsorshipFundación CRUSAes_ES
dc.description.sponsorshipUniversidad de Costa Rica/[801-A2-528]/UCR/Costa Ricaes_ES
dc.language.isoen_USes_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourcePlant Disease, vol. 92(8), pp. 1249es_ES
dc.subjectoleander leaf scorches_ES
dc.subjectnerium diseaseses_ES
dc.subjectELISAes_ES
dc.subjectPCRes_ES
dc.titleFirst Report of Xylella fastidiosa in Nerium oleander in Costa Ricaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.1094/PDIS-92-8-1249A
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional