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dc.creatorMadriz Ordeñana, Kenneth
dc.creatorRojas Montero, Roy
dc.creatorLundsgaard, Torben
dc.creatorRamírez Fonseca, Pilar
dc.creatorThordal Christensen, Hans
dc.creatorCollinge, David B.
dc.date.accessioned2019-06-28T17:29:25Z
dc.date.available2019-06-28T17:29:25Z
dc.date.issued2000-04
dc.identifier.citationhttps://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-3059.2000.00447.xes_ES
dc.identifier.issn1365-3059
dc.identifier.urihttp://hdl.handle.net/10669/77950
dc.descriptionCopyright 2000, British Society for Plant Pathology. Datos incluidos por Lisela Moreira Carmona, con autorizacion de Dr. Kenneth Madriz Ordeñana (junio 2019)es_ES
dc.description.abstractMaize rayado fino marafivirus (MRFV) was mechanically transmitted to kernels of three Costa Rican maize cultivars by the vascular puncture technique. A transmission rate of up to 43.2% was obtained for cultivar HC‐57. The rate of MRFV transmission to maize was possibly affected by the morphology of the kernel, which may influence physical access to the vascular tissue after water imbibition. Transmission to maize was confirmed by western blot and immunosorbent electron microscopy. By slight modifications of the procedure, MRFV was also transmitted to barley. This is the first report of MRFV infecting a species outside the supertribe Andropogonodae. Virus infection and replication in barley were confirmed by RT‐PCR detection of MRFV RNA and by detection of the virus by ELISAes_ES
dc.description.sponsorshipDanish International Development Agency/[]/DANIDA/Dinamarcaes_ES
dc.language.isoen_USes_ES
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.sourcePlant Pathology; vol.49(2), pp.302-307es_ES
dc.subjectBarleyes_ES
dc.subjectDalbulus maidises_ES
dc.subjectMaize rayado fino viruses_ES
dc.subjectMechanical transmissiones_ES
dc.subjectVascular puncturees_ES
dc.titleMechanical transmission of maize rayado fino marafivirus (MRFV) to maize and barley by means of the vascular puncture techniquees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.1046/j.1365-3059.2000.00447.x
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Biología Celular y Molecular (CIBCM)es_ES


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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 Internacional