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dc.creatorRey Suárez, Paola
dc.creatorSaldarriaga Córdoba, Mónica María
dc.creatorTorres Lamus, Uday Daniel
dc.creatorMarin Villa, Marcel
dc.creatorLomonte, Bruno
dc.creatorNúñez Rangel, Vitelbina
dc.date.accessioned2020-01-29T15:15:12Z
dc.date.available2020-01-29T15:15:12Z
dc.date.issued2019
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S0041010119304593?via%3Dihubes_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/80384
dc.description.abstractMicrurus mipartitus and M. dumerilii are the most medically important coral snakes in Colombia. Proteomic characterization of their venoms has previously shown that proteins of the three-finger toxin (3FTx) family are abundant components, especially in M. mipartitus (61%) and to a lesser extent in M. dumerilii (28%). In order to increase knowledge on these toxins, in this work a major 3FTx of M. dumerilii venom (8% of the venom proteins), named Clarkitoxin-I-Mdum, was isolated and characterized. Its amino acid sequence comprises 66 residues, with an isotope-averaged molecular mass of 7537 ± 2 Da and a theoretical pI of 9.36, presenting the conserved pattern of eight cysteines that classifies it as a short-chain (type I) 3FTx. Clarkitoxin-I-Mdum was not lethal to mice by intravenous or intracerebroventricular route and was not cytolytic to myogenic cells in vitro. On the other hand, five coding sequences for 3FTxs were obtained from the venom gland of M. mipartitus. These novel toxin sequences were named Mm3FTx-01 to Mm3FTx-05, all of them also presenting the eight conserved cysteines of short-chain 3FTxs. Phylogenetic analysis revealed high variability of 3FTxs from Micrurus, and ELISA using antibodies raised to the major 3FTxs from M. mipartitus and M. dumerilii confirmed their immunochemical divergence. These results highlight the relevance of performing further studies aiming at a deeper understanding of the functional and antigenic relationships among specific Micrurus toxins, with important implications for the production of antivenoms.es_ES
dc.description.sponsorshipUniversidad de Antioquia/[111577757474]/UdeA/Colombiaes_ES
dc.description.sponsorshipVicerrectoría de Investigación, Universidad de Costa Rica/[VI-741-B7608]/UCR/Costa Ricaes_ES
dc.description.sponsorshipMinisterio de medioambiente/[151 RGE: 0205]//Colombiaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon, vol.170, pp.85-93es_ES
dc.subjectMicruruses_ES
dc.subjectα-neurotoxines_ES
dc.subjectCoral snakees_ES
dc.subjectMicrurus dumeriliies_ES
dc.subjectMicrurus mipartituses_ES
dc.subjectThree-finger toxinses_ES
dc.subjectSnake venomes_ES
dc.titleNovel three-finger toxins from Micrurus dumerilii and Micrurus mipartitus coral snake venoms: phylogenetic relationships and characterization of Clarkitoxin-I-Mdumes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.1016/j.toxicon.2019.09.017
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES
dc.identifier.codproyecto741-B7-608


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