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dc.creatorSpolaore, Barbara
dc.creatorFernández Ulate, Julián
dc.creatorLomonte, Bruno
dc.creatorMassimino, Maria Lina
dc.creatorTonello, Fiorella
dc.date.accessioned2020-01-29T15:37:10Z
dc.date.available2020-01-29T15:37:10Z
dc.date.issued2019
dc.identifier.citationhttps://www.sciencedirect.com/science/article/pii/S0041010119304696?via%3Dihubes_ES
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/10669/80385
dc.description.abstractAlmost all animal venoms contain secretory phospholipases A2 (PLA2s), 14 kDa disulfide-rich enzymes that hydrolyze membrane phospholipids at the sn-2 position, releasing lysophospholipids and fatty acids. These proteins, depending on their sequence, show a wide variety of biochemical, toxic and pharmacological effects and deserve to be studied for their numerous possible applications, and to improve antivenom drugs. The cellular localization and activity of a protein can be studied by conjugating it with a tag. In this work, we applied an enzymatic labelling method, using Streptomyces mobaraense transglutaminase, on three snake venom PLA2s: a recombinant neuro- and myotoxic group I PLA2 from Notechis scutatus scutatus, and two myotoxic group II PLA2s from Bothrops asper - one of them a natural catalytically inactive variant. We demonstrate that TGase can be used to produce active mono- or bi-derivatives of these three PLA2s modified at specific Lys residues, and that all three of these proteins, conjugated with fluorescent peptides, are internalized in primary myotubes.es_ES
dc.description.sponsorshipUniversidad de Padua/[PRID2018]/PD/Italiaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon, vol.170, pp.99-107es_ES
dc.subjectSecretory phospholipase A2es_ES
dc.subjectProtein labellinges_ES
dc.subjectEnzymatic conjugationes_ES
dc.subjectTransglutaminasees_ES
dc.subjectsnake venomes_ES
dc.titleEnzymatic labelling of venom phospholipase A2 toxinses_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.1016/j.toxicon.2019.09.019
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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