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Enzymatic labelling of venom phospholipase A2 toxins
dc.creator | Spolaore, Barbara | |
dc.creator | Fernández Ulate, Julián | |
dc.creator | Lomonte, Bruno | |
dc.creator | Massimino, Maria Lina | |
dc.creator | Tonello, Fiorella | |
dc.date.accessioned | 2020-01-29T15:37:10Z | |
dc.date.available | 2020-01-29T15:37:10Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | https://www.sciencedirect.com/science/article/pii/S0041010119304696?via%3Dihub | |
dc.identifier.issn | 0041-0101 | |
dc.identifier.uri | https://hdl.handle.net/10669/80385 | |
dc.description.abstract | Almost all animal venoms contain secretory phospholipases A2 (PLA2s), 14 kDa disulfide-rich enzymes that hydrolyze membrane phospholipids at the sn-2 position, releasing lysophospholipids and fatty acids. These proteins, depending on their sequence, show a wide variety of biochemical, toxic and pharmacological effects and deserve to be studied for their numerous possible applications, and to improve antivenom drugs. The cellular localization and activity of a protein can be studied by conjugating it with a tag. In this work, we applied an enzymatic labelling method, using Streptomyces mobaraense transglutaminase, on three snake venom PLA2s: a recombinant neuro- and myotoxic group I PLA2 from Notechis scutatus scutatus, and two myotoxic group II PLA2s from Bothrops asper - one of them a natural catalytically inactive variant. We demonstrate that TGase can be used to produce active mono- or bi-derivatives of these three PLA2s modified at specific Lys residues, and that all three of these proteins, conjugated with fluorescent peptides, are internalized in primary myotubes. | es_ES |
dc.description.sponsorship | Universidad de Padua/[PRID2018]/PD/Italia | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Toxicon, vol.170, pp.99-107 | es_ES |
dc.subject | Secretory phospholipase A2 | es_ES |
dc.subject | Protein labelling | es_ES |
dc.subject | Enzymatic conjugation | es_ES |
dc.subject | Transglutaminase | es_ES |
dc.subject | snake venom | es_ES |
dc.title | Enzymatic labelling of venom phospholipase A2 toxins | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1016/j.toxicon.2019.09.019 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
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