Show simple item record

dc.creatorMolina Mora, José Arturo
dc.creatorJiménez Morgan, Sergio
dc.date.accessioned2020-02-13T16:22:56Z
dc.date.available2020-02-13T16:22:56Z
dc.date.issued2016
dc.identifier.citationhttp://www.researchpublish.com/journal/IJLSR/Issue-4-October-2016-December-2016/0es_ES
dc.identifier.issn2348-3148
dc.identifier.issn2348-313X
dc.identifier.urihttp://hdl.handle.net/10669/80580
dc.description.abstractBacterial resistance refers to bacteria capacity to evade antibiotic action, which constitutes a public health issue. This resistance is given by β-lactamase enzymes that break the drug rings and alter its function. To counteract this effect, some β-lactamase inhibitors, that have a higher affinity and irreversibly bond, have been used. However, as a consequence of selective pressure, some mutations have caused enzyme-drug affinity changes. TEM-1 is a serine- β-lactamase in which this process has been proved, giving particular interest for evaluating how these mutations affect drug-enzyme binding force. When making simulations with four mutations M182T, V184A, T160H and A224V and undertaking molecular docking, a change in the affinity pattern was observed, aiding enzyme-antibiotic bindging rather than enzyme-inhibitor bindging, which would explain lab results in which the use of β-lactamase inhibitors has not been effective. Besides, with the purpose of exploring inhibition alternatives in the enzyme, simulations with one BLIP (β-lactamase inhibitor protein) were carried out, showing that the bond between β-lactamase and BLIP alters drug access to an active site.es_ES
dc.language.isoen_USes_ES
dc.sourceInternational Journal of Life Sciences Research, vol.4(4), pp.55-61es_ES
dc.subjectTEM-1 B-lactamasees_ES
dc.subjectMolecular dockinges_ES
dc.subjectStructure simulationes_ES
dc.subjectAntibiotic resistancees_ES
dc.titleIn silico mutations of TEM-1 β-lactamase show changes in structure and drug-enzyme affinity binding by molecular dockinges_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Microbiologíaes_ES
dc.description.procedenceUCR::Vicerrectoría de Docencia::Salud::Facultad de Medicinaes_ES


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record