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dc.creatorBarquero Quirós, Mirian
dc.creatorDomínguez Renedo, Olga
dc.creatorAlonso Lomillo, María Asunción
dc.creatorArcos Martínez, María Julia
dc.date.accessioned2020-02-28T20:09:03Z
dc.date.available2020-02-28T20:09:03Z
dc.date.issued2015
dc.identifier.citationhttps://www.semanticscholar.org/paper/Biosensor-for-Aluminum(III)-Based-on-α-Chymotrypsin-Barquero-Quirós-Domínguez-Renedo/b433b1bd9f3b31e5767f8f381345194572fd67c9es_ES
dc.identifier.citationhttps://astonjournals.com/csjvols.html
dc.identifier.issn2150-3494
dc.identifier.urihttp://hdl.handle.net/10669/80664
dc.description.abstractWe report on a new amperometric assay for Al(III) ions based on the inhibition of α-chymotrypsin enzyme. The immobilization of the enzyme was performed on screen-printed carbon electrodes modified with gold nanoparticles and polyvinyl alcohol. N-acetyl-tyrosine ethyl ester, used as enzyme substrate, produces an oxidation amperometric signal, which at increasing aluminum concentration is diminished. The developed system has a detection limit of 1.5 ± 0.1 μM (n=7) for Al(III). The reproducibility of the method is 6.6% (n=5) and its repeatability is 8.2% (n=3). Main interferences at low concentration, include As(V), Cd(II), and Mo(VI) ions. The developed method was successfully applied to the determination of Al(III) in spiked white wine sample. Results agree with the certified value giving a value of percent recovery of 103 ± 4% (n=4).es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[804-B2-299]/UCR/Costa Ricaes_ES
dc.description.sponsorshipJunta de Castilla y León/[BU212A12-2]//Españaes_ES
dc.description.sponsorshipMinisterio de Ciencia e Innovación/[]/MICINN/Españaes_ES
dc.description.sponsorshipFondo Europeo de Desarrollo Regional/[INNPACTO SERIBIO 2011-2014]/FEDER/Españaes_ES
dc.description.sponsorshipFondo Europeo de Desarrollo Regional/[TEC-2009/12029]/FEDER/Españaes_ES
dc.language.isoen_USes_ES
dc.sourceChemical Sciences Journal, vol.6(2), pp.1-6es_ES
dc.subjectα-chymotrypsines_ES
dc.subjectAcetyl-L-tyrosine ethyl esteres_ES
dc.subjectBiosensores_ES
dc.subjectAluminumes_ES
dc.subjectScreen-printed electrodeses_ES
dc.subjectGold nanoparticleses_ES
dc.titleBiosensor for Aluminum(III) Based on α-Chymotrypsin Inhibition using a Disposable Screen-Printed Carbon Electrode and Acetyl-Tyrosine Ethyl Ester as Substratees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.identifier.doi10.4172/2150-3494.100089
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias Básicas::Centro de Investigación en Electroquímica y Energía Química (CELEQ)es_ES
dc.identifier.codproyecto804-B2-299


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