Deceived bilateral filter for improving the automatic cell segmentation and tracking in the NF-kB pathway without nuclear staining
artículo preliminar
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Date
2014-10-29Author
Mora Rodríguez, Rodrigo Antonio
Siles Canales, Francisco
Castro, Jorge
Sáenz Espinoza, Aránzazu
Calderón Ramírez, Saúl
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Show full item recordAbstract
In this work we implement an automatic videobased online cell tracking system for TNFa treated cells in the
transcription factor NF-kB pathway, with the aim to provide
p65 measurement approximation with time and individual cell
resolution. The video used to test our proposed tracking system
consists on cells transfected with fluorescent p65 protein. Such
protein switches its location within the cell, among the nucleus
and cytoplasm over time, causing important changes on its apparent morphology. This represents a major challenge for an automatic cell tracking system. Additionally, images in the tested
microscopy footage present noise and contrast variations. For
noise reduction, we modified the deceived bilateral filter (DBF),
a denoising and edge sharpening non linear filter. The proposed
modification of the DBF consists in an adaptive border enhancement. We tested the cell tracking algorithm with different preprocessing configurations achieving a maximum tracking accuracy improvement of 28%, with the use of the modified DBF
External link to the item
0.1007/978-3-319-13117-7_89
artículo -- Universidad de Costa Rica, Escuela de Ingeniería Eléctrica. 2014