Novel three-finger toxins from Micrurus dumerilii and Micrurus mipartitus coral snake venoms: phylogenetic relationships and characterization of Clarkitoxin-I-Mdum
artículo original
Fecha
2019Autor
Rey Suárez, Paola
Saldarriaga Córdoba, Mónica María
Torres Lamus, Uday Daniel
Marin Villa, Marcel
Lomonte, Bruno
Núñez Rangel, Vitelbina
Metadatos
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Micrurus mipartitus and M. dumerilii are the most medically important coral snakes in Colombia. Proteomic
characterization of their venoms has previously shown that proteins of the three-finger toxin (3FTx) family are
abundant components, especially in M. mipartitus (61%) and to a lesser extent in M. dumerilii (28%). In order to
increase knowledge on these toxins, in this work a major 3FTx of M. dumerilii venom (8% of the venom proteins),
named Clarkitoxin-I-Mdum, was isolated and characterized. Its amino acid sequence comprises 66 residues, with
an isotope-averaged molecular mass of 7537 ± 2 Da and a theoretical pI of 9.36, presenting the conserved
pattern of eight cysteines that classifies it as a short-chain (type I) 3FTx. Clarkitoxin-I-Mdum was not lethal to
mice by intravenous or intracerebroventricular route and was not cytolytic to myogenic cells in vitro. On the
other hand, five coding sequences for 3FTxs were obtained from the venom gland of M. mipartitus. These novel
toxin sequences were named Mm3FTx-01 to Mm3FTx-05, all of them also presenting the eight conserved cysteines
of short-chain 3FTxs. Phylogenetic analysis revealed high variability of 3FTxs from Micrurus, and ELISA
using antibodies raised to the major 3FTxs from M. mipartitus and M. dumerilii confirmed their immunochemical
divergence. These results highlight the relevance of performing further studies aiming at a deeper understanding
of the functional and antigenic relationships among specific Micrurus toxins, with important implications for the
production of antivenoms.
External link to the item
10.1016/j.toxicon.2019.09.017Colecciones
- Microbiología [1171]
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