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Ten simple rules for aspiring scientists in a low-income country 

Gutiérrez, José María; Moreno Robles, Edgardo (PLOS Computational Biology, vol. 4, n. 5, e1000024, 2008, 2008)
Being a scientist entails a common set of characteristics. Admiring nature and having concern for social issues; possessing a strong academic background, team work abilities, honesty, discipline, skepticism, communication ...
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Confronting the neglected problem of snake bite envneoming: the need for a global partnership 

Gutiérrez, José María; Theakston, R. David G.; Warrell, David A. (PLoS Medicine, 2006)
Envenoming resulting from snake bites is an important public health hazard in many regions, particularly in tropical and subtropical countries [1–3]. Although antivenoms are being produced by various laboratories in every ...

Autocatalytic acylation of phospholipase-like myotoxins 

Pedersen, J. Z.; Lomonte, Bruno; Massoud, R.; Gubensek, F.; Gutiérrez, José María; Rufini, Stefano (1995-04)
Several snake venoms contain a phospholipase A2 in which position 49 in the active site is occupied by a lysine or a serine instead of the aspartate residue normally found. Although these proteins do not bind Ca2+ and are ...

Isolation from a polyvalent antivenom of antibodies to a myotoxin in Bothrops asper snake venom 

Lomonte, Bruno; Mata, Eugenia; Gutiérrez, José María (1985)
Antibodies against Bothrops asper rmyotoxin were purified from a polyvalent antivenom by affinity chromatography . These antibodies neutralized most of the myotoxic activity of crude B. asper venom, as judged by histologic ...

Neutralization of myotoxic phospholipases A2 from the venom of the snake Bothrops asper by monoclonal antibodies 

Lomonte, Bruno; Gutiérrez, José María; Ramírez Pacheco, Margarita; Díaz Oreiro, Cecilia (1992)
The neutralization of two myotoxic phospholipases A2 from the venom of Bothrops asper, myotoxins I and II, by two murine monoclonal antibodies is reported. The monoclonal antibodies, MAb-3 and Mab-4, recognize different ...

Pathological and biochemical changes induced in mice after intramuscular injection of venom from newborn specimens of the snake Bothrops asper (Terciopelo) 

Chaves Mora, Fernando; Gutiérrez, José María; Brenes Pino, Fernando (1992-09)
Venom from newborn Bothrops asper snakes has higher lethal, hemorrhagic, edema-forming, proteolytic and defibrinating activities than venom from adult B. asper specimens. Electrophoretic analysis confirmed the variation ...
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Neutralización de las actividades tóxicas y enzimáticas de cuatro venenos de serpientes de Guatemala y Honduras por el antiveneno polivalente producido en Costa Rica 

Rojas Céspedes, Gustavo; Gutiérrez, José María; Gené, José Antonio; Gómez Paniagua, Maribel; Cerdas Fallas, Luis (1987)
We studied the ability of the polyvalent antivenom produced in Costa Rica to neutralize lethal, hemorrhagic, edema-forming, proteolytic, hemolytic, hyaluronidase and fibrinolytic activities of the venoms of Bothrops asper ...
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Evaluation of antivenoms in the neutralization of hyperalgesia and edema induced by Bothrops jararaca and Bothrops asper snake venoms 

Picolo, Gisele; Chacur, Marucia; Gutiérrez, José María; Teixeira, Catarina de Fátima; Cury, Yara (2002-10)
Neutralization of hyperalgesia induced by Bothrops jararaca and B. asper venoms was studied in rats using bothropic antivenom produced at Instituto Butantan (AVIB, 1 ml neutralizes 5 mg B. jararaca venom) and polyvalent ...

Elapid venom toxins: multiple recruitments of ancient scaffolds 

Alape Girón, Alberto; Persson, Bengt; Cederlund, Ella; Flores Díaz, Marietta; Gutiérrez, José María; Thelestam, Mónica; Bergman, Tomas; Jörnvall, Hans (1999-01)
Nigroxins A and B, two myotoxic phospholipases A2 (PLA2s) from the venom of the American elapid Micrurus nigrocinctus, belong to a new PLA2 subclass. Their primary structures were established and compared with those of ...

Danger in the reef: Proteome, toxicity, and neutralization of the venom of the olive sea snake, Aipysurus laevis 

Laustsen, Andreas Hougaard; Gutiérrez, José María; Rasmussen, Arne R.; Engmark, Mikael Gerling; Gravlund, Peter; Sanders, Kate L.; Lohse, Brian; Lomonte, Bruno (2015-12-01)
Four specimens of the olive sea snake, Aipysurus laevis, were collected off the coast of Western Australia, and the venom proteome was characterized and quantitatively estimated by RP-HPLC, SDS-PAGE, and MALDI-TOF-TOF ...
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