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Cleavage of the NH2-Terminal Octapeptide of Bothrops asper Myotoxic Lysine-49 Phospholipase A2 Reduces Its Membrane-Destabilizing Effect 

Díaz Oreiro, Cecilia; Alape Girón, Alberto; Lomonte, Bruno; Olamendi Portugal, Timoteo; Gutiérrez, José María (1994-08)
Bothrops asper myotoxin II was cleaved with cyanogen bromide to determine the role of NH2-terminal amino acid residues in its ability to destabilize negatively charged liposomes and to induce myonecrosis. After treatment, ...

Dissociation of enzymatic and toxic activities by the use of antibodies
Disociación de las actividades enzimáticas y tóxicas mediante la utilización de anticuerpos 

Lomonte, Bruno; Gutiérrez, José María (1990)
IN A RECENT article KASTURi and GOWDA,(1990) described the dissociation of enzymatic and toxic activities of phospholipases A, (PLA) from Vipera russelli venom by neutraliza- tion experiments u ...

Caprylic acid fractionation of hyperimmune horse plasma: description of a simple procedure for antivenom production 

Rojas Céspedes, Gustavo; Jiménez, José Manuel; Gutiérrez, José María (1994)
A simple methodology for hyperimmune horse plasma fractionation, based on caprylic acid precipitation, is described. Optimal conditions for fractionation were studied; the method gives best results when concentrated caprylic ...

A potent antihemorrhagin in the serum of the non-poisonous water snake Natrix tessellata: isolation, characterization and mechanism of neutralization 

Borkow, Gadi; Gutiérrez, José María; Ovadia, Michael (1994-12)
The main natural antihemorrhagic factor (NtAH), which inhibits the hemorrhagic activity of Bothrops asper snake venom, was isolated from the serum of the non-poisonous water snake Natrix tessellata by ammonium sulfate ...

Isolation and characterization of a metalloproteinase with weak hemorrhagic activity from the venom of the snake Bothrops asper (terciopelo) 

Gutiérrez, José María; Romero, Marjorie; Díaz Oreiro, Cecilia; Borkow, Gadi; Ovadia, Michael (1995-01)
A metalloproteinase, named BaP1, was purified to homogeneity from the venom of Bothrops asper (Pacific region) of Costa Rica by ion-exchange chromatography on CM-Sephadex and gel filtration on Sephacryl S-200. The enzyme ...

The amino acid sequence of a myotoxic phospholipase from the venom of Bothrops asper 

Kaiser, Ivan I.; Gutiérrez, José María; Plummer, Dorothy; Aird, Steven Douglas; Odell, George V. (1990)
A myotoxic, basic phospholipase A2 (pI greater than 9.5) with anticoagulant activity has been purified from the venom of Bothrops asper, and its amino acid sequence determined by automated Edman degradation. It is distinct ...

Snake venomics and antivenomics of Bothrops atrox venoms from Colombia and the Amazon regions of Brazil, Perú and Ecuador suggest the occurrence of geographic variation of venom phenotype by a trend towards paedomorphism 

Núñez Rangel, Vitelbina; Cid, Pedro; Sanz, Libia; De La Torre, Pilar; Angulo Ugalde, Yamileth; Lomonte, Bruno; Gutiérrez, José María; Calvete Chornet, Juan José (2009-11-02)
The venom proteomes of Bothrops atrox from Colombia, Brazil, Ecuador, and Perú were characterized using venomic and antivenomic strategies. Our results evidence the existence of two geographically differentiated venom ...
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Hacia una mayor integración de las ciencias naturales y las ciencias sociales: análisis de un caso relacionado con un problema de salud pública 

Gutiérrez, José María (2009)
Se discute la conveniencia de promover una interacción y un diálogo productivo entre las ciencias naturales, las ciencias sociales y las tecnologías. El tema se analiza alrededor del caso de un problema de salud: los ...
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A Lys49 Phospholipase A2, Isolated from Bothrops asper Snake Venom, Induces Lipid Droplet Formation in Macrophages Which Depends on Distinct Signaling Pathways and the C-Terminal Region 

Giannotti, Karina Cristina; Leiguez, Elbio; Moreira, Vanessa; Galvão Nascimento, Neide; Lomonte, Bruno; Gutiérrez, José María; Lopes de Melo, Robson; Teixeira, Catarina de Fátima (2013)
MT-II, a Lys49PLA2 homologue devoid of catalytic activity from B. asper venom, stimulates inflammatory events in macrophages. We investigated the ability of MT-II to induce formation of lipid droplets (LDs), key elements ...

Role of cyclooxygenases in oedema-forming activity of bothropic venoms 

Olivo, Renata do A.; Teixeira, Catarina de Fátima; Wallace, John L.; Gutiérrez, José María; Zamunér, Stella Regina (2007-04)
The venoms of Bothrops asper (BaV) and Bothrops jararaca (BjV), two of the most medically important poisonous snakes of Latin America, cause pronounced oedema in the victims through poorly understood mechanisms. In the ...
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Gutiérrez, José María (399)
Lomonte, Bruno (177)Rucavado Romero, Alexandra (64)Calvete Chornet, Juan José (57)León Montero, Guillermo (53)Angulo Ugalde, Yamileth (46)Sanz, Libia (43)Herrera Vega, María (36)Rojas Céspedes, Gustavo (34)Escalante Muñoz, Teresa (33)... View MoreTypeartículo científico (385)capítulo de libro (9)contribución a revista (2)libro (1)otro (1)preprint (1)ProcedenceVicerrectoría de Investigación (398)Vicerrectoría de Docencia (32)... View MoreHas File(s)Yes (397)No (2)

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