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Microflora on bean seeds (Phaseolus vulgaris L.).

dc.creatorMembreño, José B.
dc.creatorZapata, Mildred
dc.creatorBeaver, James S.
dc.creatorSmith, Rusty
dc.date2014-11-26
dc.date.accessioned2016-05-02T20:55:47Z
dc.date.available2016-05-02T20:55:47Z
dc.identifierhttp://revistas.ucr.ac.cr/index.php/agromeso/article/view/17224
dc.identifier.urihttps://hdl.handle.net/10669/17881
dc.descriptionSe estudió la microflora bacteriana presente en semillas de frijol y su relación con Xanthomonas campestris pv. phaseoli (Xcp), en 118 genotipos procedentes de VIDAC-98, INTA- Nicaragua, TARS-USDA e Isabela-P.R. Se utilizaron cinco métodos de aislamiento: semilla desinfectada con hipoclorito de sodio, semilla en caldo nutritivo refrigerada por una hora, dispersión de 0,1 ml de suspensión de semillas en medio sólido, siembra líquida de 1 ml de suspensión y semilla en caldo nutritivo, agitado y refrigerado por 24 horas. Se aislaron 104 colonias amarillas de 41 genotipos. Treinta y seis colonias fueron KOH positivo (Gram negativo), 68 negativo (Gram positivo) y 34 hidrolizaron almidón. Las colonias de pigmentación amarilla resultaron no patogénicas bajo condiciones de invernadero. Estas se identificaron con el sistema BIOLOG como: Pantoea agglomerans (25), Xanthomonas campestris (2), Enterobacter agglomerans (2), Sphingomonas paucimobilis (2), Pseudomonas fluorescens y Flavimonas oryzihabitans. En adición, los genotipos portaron colonias con pigmentación distinta a la amarilla. En las pruebas de antagonismo se identificaron colonias con actividad de deoxyribonucleasa y de antibiosis a Xcp. De éstas, 15 colonias inhibieron a Xcp significativamente. Se identificaron los hongos Rhizoctonia solani, Penicillium spp., Fusarium spp., Aspergillus flavus, Rhizopus nigricans y Macrophomina phaseolina en un 52,9 % del total de genotipos evaluados.es-ES
dc.descriptionThe bacterial microflora present and its relationship with Xanthomonas campestris pv. phaseoli (Xcp) were studied in bean seeds, on 118 genotypes coming from VIDAC - 98, INTA - Nicaragua, TARS - USDA and Isabela - P.R. Five isolation methods were used: seed decontaminated with sodium hypochlorite, seed treated with refrigerated nutrient broth for one hour, dispersion of 0.1 ml of suspension of seeds between solid, liquid sowing of 1 ml suspension and seed in nutritious, agitated broth and refrigerated by 24 hours. One hundred and four yellow colonies from 41 genotypes were isolated. Thirty-six colonies were positive KOH (Gram negative), 68 negative (Gram positive) and 34 were starch hydrolyzers. The yellow colonies were nonpathogenic under greenhouse conditions. These were identified with the BIOLOG system as: Pantoea agglomerans (25), Xanthomonas campestris (2), Enterobacter agglomerans (2), Sphingomonas paucimobilis (2), Pseudomonas fluorescens and Flavimonas oryzihabitans. In addition, the genotypes carried colonies with other pigmentations. Antagonistic colonies were identified with deoxyribonuclease activity and antibiosis to Xcp. Of these, 15 colonies inhibited Xcp significantly. The fungi were identified as: Rhizoctonia solani, Penicillium spp., Fusarium spp., Aspergillus flavus, Rhizopus nigricans and Macrophomina phaseolina in 52.9% of the total evaluated genotypes.en-US
dc.formatapplication/pdf
dc.languagespa
dc.publisherUniversidad de Costa Ricaes-ES
dc.rightsCopyright (c) 2014 Agronomía Mesoamericanaes-ES
dc.sourceMesoamerican Journal of Agronomy; Agronomía Mesoamericana: Vol 12, Nº 2; 135-139en-US
dc.sourceAgronomía Mesoamericana; Agronomía Mesoamericana: Vol 12, Nº 2; 135-139es-ES
dc.sourceAgronomía Mesoamericana; Agronomía Mesoamericana: Vol 12, Nº 2; 135-139pt-PT
dc.source2215-3608
dc.source1021-7444
dc.titleMicroflora en semillas de frijol (Phaseolus vulgaris L.).es-ES
dc.titleMicroflora on bean seeds (Phaseolus vulgaris L.).en-US
dc.typeartículo original


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