Listar Microbiología por autor "65a6da9d-1539-49b2-82a1-1878151356d9"
Mostrando ítems 1-10 de 10
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Activity of hemorrhagic metalloproteinase BaH-1 and myotoxin II from Bothrops asper snake venom on capillary endothelial cells in vitro
Lomonte, Bruno; Gutiérrez, José María; Borkow, Gadi; Ovadia, Michael; Tarkowski, Andrej; Hanson, Lars Ake (1994-04)In vivo, hemorrhagic toxins isolated from snake venoms cause a disorganization of the basal lamina of capillaries, with a concomitant degenerative process of endothelial cells. In this study we investigated the effects of ... -
Acute-phase reactions, including cytokines, in patients bitten by Bothrops and Crotalus snakes in Brazil
Barraviera, Benedito; Lomonte, Bruno; Tarkowski, Andrej; Hanson, Lars Ake; Meira, D. A. (1995)Thirty-one patients bitten by venomous snakes in Botucatu area (State of São Paulo - Brazil), sixteen by Bothrops spp. and fifteen by Crotalus durissus terrificus, were studied. The group comprised twenty-nine males and ... -
An MTT-based method for the in vivo quantification of myotoxic activity of snake venoms and its neutralization by antibodies
Lomonte, Bruno; Gutiérrez, José María; Romero, Marjorie; Núñez, Javier; Tarkowski, Andrej; Hanson, Lars Ake (1993-05)The reduction of the tetrazolium compound MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) was used as the basis for the development of a simple method for the quantitative estimation of metabolically ... -
Biological and biochemical activities of Vipera berus (European viper) venom
Calderón Fuentes, Leonel; Lomonte, Bruno; Gutiérrez, José María; Tarkowski, Andrej; Hanson, Lars Ake (1993-06)Vipera berus is widely distributed throughout the northern part of Europe and Asia. Characterization of several toxic effects of its venom in the mouse, as well as of in vitro enzymatic activities was performed. Vipera ... -
Broad cytolytic specificity of myotoxin II, a lysine-49 phospholipase A2 of Bothrops asper snake venom
Lomonte, Bruno; Tarkowski, Andrej; Hanson, Lars Ake (1994)The cytotoxic activity of Bothrops asper myotoxin II, a lysine-49 phospholipase A2 isoform, on different cell types in culture, was investigated. Myotoxin II caused a dose-dependent cytolytic effect on all cell types tested, ... -
Host response to Bothrops asper snake venom: analysis of edema formation, inflammatory cells, and cytokine release in a mouse model
Lomonte, Bruno; Tarkowski, Andrej; Hanson, Lars Ake (1993)As part of tle characterization of the host reactivity to the venom of Bothrops asper, we investigated the inflammatory responses in the mouse footpad model. The subcutaneously iniected venom induced a rapid increase of ... -
Neutralization of the cytolytic and myotoxic activities of phospholipases A2 from Bothrops asper snake venom by glycosaminoglycans of the heparin/heparan sulfate family
Lomonte, Bruno; Tarkowski, Andrej; Bagge, Ulf; Hanson, Lars Ake (1994)Basic phospholipases A2 from the venom of Bothrops asper exhibit skeletal muscle damaging activity in vivo, and cytolytic activity to a variety of cell types in culture. Glycosaminoglycans of the heparin/heparan sulfate ... -
Neutralizing interaction between heparins and myotoxin II, a Lys-49 phospholipase A2 from Bothrops asper snake venom. Identification of a heparin-binding and cytolytic toxin region by the use of synthetic peptides and molecular modeling
Lomonte, Bruno; Moreno Frias, Ernesto; Tarkowski, Andrej; Hanson, Lars Ake; Maccarana, Marco (1994)Heparin binds to phospholipase A2 myotoxins from Bothrops asper snake venom, inhibiting their toxic activities. This interaction was investigated using purified myotoxin II, a Lys-49 phospholipase A2 of this venom, and a ... -
Phospholipase A2 and inflammation
Lomonte, Bruno; Tarkowski, Andrej; Hanson, Lars Ake (1995)Increased expression of secreted group II phospholipase A 2 (sPLA2) is associated with inflammation 1.This enzyme can promote a variety of potentially harmful events, which have been attributed to its catalytic activity. ... -
Similar effectiveness of Fab and F(ab')2 antivenoms in the neutralization of hemorrhagic activity of Vipera berus snake venom in mice
Lomonte, Bruno; León Montero, Guillermo; Hanson, Lars Ake (1996)The ability of two antivenoms to Vipera spp., consisting of Fab (Therapeutic Antibodies), or of F(ab′)2 (Zagreb Institute of Immunology) antibody fragments, to neutralize the hemorrhagic activity of Vipera berus snake venom ...