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Neutralization of myotoxic phospholipases A2 from the venom of the snake Bothrops asper by monoclonal antibodies
dc.creator | Lomonte, Bruno | |
dc.creator | Gutiérrez, José María | |
dc.creator | Ramírez Pacheco, Margarita | |
dc.creator | Díaz Oreiro, Cecilia | |
dc.date.accessioned | 2016-11-10T14:45:22Z | |
dc.date.available | 2016-11-10T14:45:22Z | |
dc.date.issued | 1992 | |
dc.identifier.issn | 0040-0101 | |
dc.identifier.uri | https://hdl.handle.net/10669/29173 | |
dc.description.abstract | The neutralization of two myotoxic phospholipases A2 from the venom of Bothrops asper, myotoxins I and II, by two murine monoclonal antibodies is reported. The monoclonal antibodies, MAb-3 and Mab-4, recognize different epitopes of the toxin. Both antibodies completely neutralized myotoxic activity of myotoxin I and crude venom. MAb-3 also completely neutralized myotoxic activity of myotoxin II, a lysine-49 phospholipase A2 isoform, whereas MAb-4 neutralized this toxin only partially. MAb-3 neutralized myotoxin II at a molar ratio of 1:1, showing a higher efficiency than affinity-purified polyclonal antibodies. A dissociation of myotoxic and enzymatic activities of myotoxin I was observed with both monoclonal antibodies. | es_ES |
dc.language.iso | en_US | es_ES |
dc.source | Toxicon. Volumen 30, Número 3, 1992 | es_ES |
dc.subject | Animals | es_ES |
dc.subject | Antibodies | es_ES |
dc.subject | Monoclonal | es_ES |
dc.subject | Crotalid Venoms | es_ES |
dc.subject | Epitopes | es_ES |
dc.subject | Group II Phospholipases A2 | es_ES |
dc.subject | Mice | es_ES |
dc.subject | Inbred BALB C | es_ES |
dc.subject | Neurotoxins | es_ES |
dc.subject | Neutralization Tests | es_ES |
dc.subject | Phospholipases A | es_ES |
dc.subject | Phospholipases A2 | es_ES |
dc.subject | Reptilian Proteins | es_ES |
dc.title | Neutralization of myotoxic phospholipases A2 from the venom of the snake Bothrops asper by monoclonal antibodies | es_ES |
dc.type | artículo original | |
dc.identifier.doi | 10.1016/0041-0101(92)90866-4 | |
dc.description.procedence | UCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP) | es_ES |
dc.description.procedence | UCR::Vicerrectoría de Docencia::Salud::Facultad de Medicina::Escuela de Medicina | es_ES |
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