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dc.creatorGutiérrez, José María
dc.creatorSanz, Libia
dc.creatorEscolano, José
dc.creatorFernández Ulate, Julián
dc.creatorLomonte, Bruno
dc.creatorAngulo Ugalde, Yamileth
dc.creatorRucavado Romero, Alexandra
dc.creatorWarrell, David A.
dc.creatorCalvete Chornet, Juan José
dc.date.accessioned2016-11-29T15:23:05Z
dc.date.available2016-11-29T15:23:05Z
dc.date.issued2008-10
dc.identifier.citationhttp://pubs.acs.org/doi/abs/10.1021/pr8003826
dc.identifier.issn1535-3907
dc.identifier.urihttps://hdl.handle.net/10669/29334
dc.description.abstractThe venom proteomes of the snakes Bothrops caribbaeus and Bothrops lanceolatus, endemic to the Lesser Antillean islands of Saint Lucia and Martinique, respectively, were characterized by reversephase HPLC fractionation, followed by analysis of each chromatographic fraction by SDS-PAGE, N-terminal sequencing, MALDI-TOF mass fingerprinting, and collision-induced dissociation tandem mass spectrometry of tryptic peptides. The venoms contain proteins belonging to seven (B. caribbaeus) and five (B. lanceolatus) types of toxins. B. caribbaeus and B. lanceolatus venoms contain phospholipases A2, serine proteinases, L-amino acid oxidases and zinc-dependent metalloproteinases, whereas a long disintegrin, DC-fragments and a CRISP molecule were present only in the venom of B. caribbaeus, and a C-type lectin-like molecule was characterized in the venom of B. lanceolatus. Compositional differences between venoms among closely related species from different geographic regions may be due to evolutionary environmental pressure acting on isolated populations. The venoms of these two species differed in the composition and the relative abundance of their component toxins, but they exhibited similar toxicological and enzymatic profiles in mice, characterized by lethal, hemorrhagic, edemaforming, phospholipase A2 and proteolytic activities. The venoms of B. caribbaeus and B. lanceolatus are devoid of coagulant and defibrinogenating effects and induce only mild local myotoxicity in mice. The characteristic thrombotic effect described in human envenomings by these species was not reproduced in the mouse model. The toxicological profile observed is consistent with the abundance of metalloproteinases, PLA2s and serine proteinases in the venoms. A polyvalent (Crotalinae) antivenom produced in Costa Rica was able to immunodeplete ∼80% of the proteins from both B. caribbaeus and B. lanceolatus venoms, and was effective in neutralizing the lethal, hemorrhagic, phospholipase A2 and proteolytic activities of these venomses_ES
dc.description.sponsorshipUniversidad de Costa Rica/[741-A8-521]/UCR/Costa Ricaes_ES
dc.description.sponsorshipMinisterio de Educación y Ciencia/[BFU2004-01432/BMC]//Españaes_ES
dc.description.sponsorshipMinisterio de Educación y Ciencia/[BFU2007-61563]//Españaes_ES
dc.description.sponsorshipConsejo Superior de Investigaciones Científicas/2007CR0004/CRUSA-CSIC/Españaes_ES
dc.description.sponsorshipPrograma Iberoamericano de Ciencia y Tecnología para el Desarrollo/206AC0281/CYTED/Españaes_ES
dc.language.isoen_USes_ES
dc.rightsacceso embargado
dc.sourceJournal of Proteome Research; Volumen 7, Número 10. 2008es_ES
dc.subjectBothrops Caribbaeuses_ES
dc.subjectBothrops lanceolatuses_ES
dc.subjectSaint Lucia lancehead viperes_ES
dc.subjectFer-de-lance pitviperes_ES
dc.subjectImmunodepletiones_ES
dc.subjectMass spectrometryes_ES
dc.subjectN-terminal Sequencinges_ES
dc.subjectPolyvalent (Crotalinae) Antivenomes_ES
dc.subjectProteomicses_ES
dc.subjectSnake venom protein familieses_ES
dc.subjectSnake venomicses_ES
dc.subjectviperid toxinses_ES
dc.subjectSnake venomes_ES
dc.titleSnake Venomics of the Lesser Antillean Pit Vipers Bothrops caribbaeus and Bothrops lanceolatus: Correlation with Toxicological Activities and Immunoreactivity of a Heterologous Antivenomes_ES
dc.typeartículo original
dc.identifier.doi10.1021/pr8003826
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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