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dc.creatorAlape Girón, Alberto
dc.creatorMiranda Arrieta, Keyna
dc.creatorCortés Bratti, Ximena
dc.creatorStiles, Bradley G.
dc.creatorGutiérrez, José María
dc.date.accessioned2016-12-14T21:37:15Z
dc.date.available2016-12-14T21:37:15Z
dc.date.issued1997-04
dc.identifier.citationhttp://www.sciencedirect.com/science/article/pii/S004101019600150X
dc.identifier.issn0041-0101
dc.identifier.urihttps://hdl.handle.net/10669/29401
dc.description.abstractTherapeutic antisera against Micrurus nigrocinctus venom were tested for protection against lethality, as well as for inhibition of the nicotinic acetylcholine receptor (AchR)-binding and neutralization of phospholipase A2 (PLA2) activities of the homologous venom. Protection against venom lethality did not correlate with inhibition of AchR-binding activity, whereas there was a significant correlation between antisera potency and inhibition of PLA2 activity (r = 0.82, n = 10, P < 0.02). Inhibition of PLA2 activity could be useful in assessing the protective efficacy of M. nigrocinctus antisera during antivenom production. Micrurus nigrocinctus nigrocinctus venom proteins were fractionated by cation-exchange chromatography on Mono S FPLC and fractions assayed for lethality, AchR-binding and PLA2 activities. Antisera were titrated by enzyme-linked immunoassay (ELISA) against a crude M. n. nigrocinctus venom, two FPLC lethal fractions containing AchR-binding activity, and two toxins purified from M. n. nigrocinctus venom. No correlation was found between protective efficacy and the ELISA titer against any of these antigens. Compared to other elapid venoms that contain few toxins as major components, M. n. nigrocinctus venom appears to be more complex and its lethal effect is likely to be due to the combined effect of several neurotoxins.es_ES
dc.description.sponsorshipUniversidad de Costa Rica/[74l-93-301]/UCR/Costa Ricaes_ES
dc.description.sponsorshipInternational Foundation for Science/[F/0883-4]/IFS/Sueciaes_ES
dc.description.sponsorshipSwedish Agency for Research Cooperation with Developing Countries//SAREC/Sueciaes_ES
dc.language.isoen_USes_ES
dc.sourceToxicon; Volumen 35, Número 4, 1997es_ES
dc.subjectAnimalses_ES
dc.subjectAntiveninses_ES
dc.subjectElapid Venomses_ES
dc.subjectElapidaees_ES
dc.subjectEnzyme-Linked Immunosorbent Assayes_ES
dc.subjectMicees_ES
dc.subjectPhospholipases Aes_ES
dc.subjectPhospholipases A2es_ES
dc.subjectReceptors, Nicotinices_ES
dc.subjectSnake Biteses_ES
dc.subjectSnake venomes_ES
dc.titleA comparison of in vitro methods for assessing the potency of therapeutic antisera against the venom of the coral snake Micrurus nigrocinctuses_ES
dc.typeartículo científico
dc.identifier.doi10.1016/S0041-0101(96)00150-Xes_ES
dc.description.procedenceUCR::Vicerrectoría de Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto Clodomiro Picado (ICP)es_ES


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